{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR786/009/SRR7868099/SRR7868099.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR786/000/SRR7868090/SRR7868090.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR786/007/SRR7868097/SRR7868097.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR786/008/SRR7868098/SRR7868098.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR786/004/SRR7868094/SRR7868094.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR786/005/SRR7868095/SRR7868095.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR786/003/SRR7868093/SRR7868093.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR786/002/SRR7868092/SRR7868092.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR786/001/SRR7868091/SRR7868091.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR786/009/SRR7868089/SRR7868089.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR786/006/SRR7868096/SRR7868096.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR786/000/SRR7868100/SRR7868100.fastq.gz"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Case Western Reserve University"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA491649"],"scientific_name":["Mus musculus"],"tag":["xref:PubMed:30380414"],"long_description":["The RNA binding protein Dazl is essential for gametogenesis, but its direct in vivo functions, RNA targets, and the molecular basis for germ cell loss in DAZL null mice are unknown. Here, we mapped transcriptome-wide Dazl-RNA interactions in vivo, revealing Dazl binding to thousands of mRNAs via polyA-proximal 3?UTR interactions. In parallel, fluorescence activated cell sorting and RNA-Seq identified mRNAs sensitive to Dazl deletion in male germ cells. Despite binding a broad set of mRNAs, integrative analyses indicate that Dazl post-transcriptionally controls only a subset of its mRNA targets, namely those corresponding to a network of genes critical for germ cell proliferation and survival. Additionally, we provide evidence that polyA sequences have key roles in specifying Dazl-RNA interactions across the transcriptome. Altogether, our results reveal a mechanism for Dazl-RNA binding, and illustrate that Dazl functions as a master regulator of a post-transcriptional mRNA program essential for germ cell survival. Overall design: Ribosome Profiling and RNA-seq libraries from monoclonal cell line with doxycyline inducible Dazl expression (GC-1-spg parental line)"],"repository":["ENA"],"description_synonyms":["OLM4, DNA polalpha, Footprintings, GIANT CHLOROPLAST 1, dazl1, Capture, Daz-like, ssq, pol alpha, Gm296, RNA-seq., Viabilities, DNA pol-73, familial spastic paraparesis, polalpha, CG5923, DNApola, CG11754, Myo, FSP, Translation, polalpha50, Translation Profilings, DmelCG7108, Messenger, UNQ362, Pol, Ribosome Footprinting, Survival, dnapol alpha 50, Ribo-Seq, mat3(6), AAB24152, CG6349, NSX, GW112, hGC-1, Whole Transcriptome Shotgun Sequencing, MLRC, Profilings, CG3595, Non Polyadenylated, Viability, E(mus304), dazh, Ribosome-Bound tRNA Captures, RLC, p180, Polalpha 73kDa, Myo-II, DNApol-alpha, Polyadenylated Messenger, Gm913, dazl-B, Ribosome-Bound tRNA Capture, messenger RNA, DmPolalpha, Polalpha, tRNA Capture, spgyla, Dazla, CG14530, swi7, template RNA, pPD4, POlalpha 180kDa, bA209J19.1, DmMRLC_C, DNApolalpha50, Dazl1, Dm Dock4, DAZLA, NMM, Tpx2, DAZH, ATGC1, Myo II, dazla, hOLfD, OlfD, DAZL1, pola, DmelCG3595, Translation Profiling, EIEE3, RGD1307826, Profiling, DNApolalpha, RNA, DmelCG6349, DNApol, guanylyl cyclase 1, MLC, DNA Pol-alpha180, ARTSeq, K18J17.8, Polyadenylated, Ribosome Profilings, CG7108, POLA, AW321876, Messenger RNA, ribosome profiling, sqh/RLC, sgh, active mRNA translation sequencing, myoII, Cell, Dmpol alpha, polA, Cell Viabilities, Ribosome-Bound tRNA, DmelCG5923, Dazh, spg, Sqh, Poly(A)+ mRNA, polalphap180, PRI1, INSDC_feature:mRNA, Footprinting, ARABIDOPSIS GUANYLYL CYCLASE 1, Gc1, Polyadenylated RNA, Squh, Squ, dazl-a, tRNA Captures, Ribosome Bound tRNA Capture, Translating Ribosome Affinity Purification, ATSULA, anon-WO0140519.257, Xdazl, Polyadenylated Messenger RNA, 183 kDa pol alpha, Ribosome, Poly(A)+ RNA, Polalpha 50kDa, Non Polyadenylated mRNA, protein_coding_transcript, mRNA, Poly(A) Tail, DNApol-alpha72, Non-Polyadenylated, GC1, RMLC, Tpx-2, NET44, squ, Dpri50, mat(3)6, Ribosome-Bound, Non-Polyadenylated mRNA, Ribosome Footprintings, Pol alpha, DNApolalpha180, Captures, SPGYLA, DmelCG31048, ribosome footprinting, Pola, 1300006L01Rik, DNA-Polalpha, K18J17_8, MyoII, MRLC, Polyadenylated mRNA, AI060884, Cell Viability, Poly(A) RNA, CG31048"],"name_synonyms":["OLM4, DNA polalpha, Footprintings, GIANT CHLOROPLAST 1, dazl1, Capture, Daz-like, ssq, pol alpha, Gm296, RNA-seq., Viabilities, DNA pol-73, familial spastic paraparesis, polalpha, CG5923, DNApola, CG11754, Myo, FSP, Translation, polalpha50, Translation Profilings, DmelCG7108, Messenger, UNQ362, Pol, Ribosome Footprinting, Survival, dnapol alpha 50, Ribo-Seq, mat3(6), AAB24152, CG6349, NSX, GW112, hGC-1, Whole Transcriptome Shotgun Sequencing, MLRC, Profilings, CG3595, Non Polyadenylated, Viability, E(mus304), dazh, Ribosome-Bound tRNA Captures, RLC, p180, Polalpha 73kDa, Myo-II, DNApol-alpha, Polyadenylated Messenger, Gm913, dazl-B, Ribosome-Bound tRNA Capture, messenger RNA, DmPolalpha, Polalpha, tRNA Capture, spgyla, Dazla, CG14530, swi7, template RNA, pPD4, POlalpha 180kDa, bA209J19.1, DmMRLC_C, DNApolalpha50, Dazl1, Dm Dock4, DAZLA, NMM, Tpx2, DAZH, ATGC1, Myo II, dazla, hOLfD, OlfD, DAZL1, pola, DmelCG3595, Translation Profiling, EIEE3, RGD1307826, Profiling, DNApolalpha, RNA, DmelCG6349, DNApol, guanylyl cyclase 1, MLC, DNA Pol-alpha180, ARTSeq, K18J17.8, Polyadenylated, Ribosome Profilings, CG7108, POLA, AW321876, Messenger RNA, ribosome profiling, sqh/RLC, sgh, active mRNA translation sequencing, myoII, Cell, Dmpol alpha, polA, Cell Viabilities, Ribosome-Bound tRNA, DmelCG5923, Dazh, spg, Sqh, Poly(A)+ mRNA, polalphap180, PRI1, INSDC_feature:mRNA, Footprinting, ARABIDOPSIS GUANYLYL CYCLASE 1, Gc1, Polyadenylated RNA, Squh, Squ, dazl-a, tRNA Captures, Ribosome Bound tRNA Capture, Translating Ribosome Affinity Purification, ATSULA, anon-WO0140519.257, Xdazl, Polyadenylated Messenger RNA, 183 kDa pol alpha, Ribosome, Poly(A)+ RNA, Polalpha 50kDa, Non Polyadenylated mRNA, protein_coding_transcript, mRNA, Poly(A) Tail, DNApol-alpha72, Non-Polyadenylated, GC1, RMLC, Tpx-2, NET44, squ, Dpri50, mat(3)6, Ribosome-Bound, Non-Polyadenylated mRNA, Ribosome Footprintings, Pol alpha, DNApolalpha180, Captures, SPGYLA, DmelCG31048, ribosome footprinting, Pola, 1300006L01Rik, DNA-Polalpha, K18J17_8, MyoII, MRLC, Polyadenylated mRNA, AI060884, Cell Viability, Poly(A) RNA, CG31048"],"additional_accession":[]},"is_claimable":false,"name":"Dazl regulates germ cell survival through a network of polA-proximal mRNA interactions [GC1-spg cells (parental line) Ribosome Profiling and RNA-seq]","description":"Dazl regulates germ cell survival through a network of polA-proximal mRNA interactions [GC1-spg cells (parental line) Ribosome Profiling and RNA-seq]","dates":{"last_updated":"2025-09-24","first_public":"2018-09-21"},"accession":"PRJNA491649","cross_references":{"GEO":["GSE120097"],"taxon":["10090"],"PubMed":["30380414"]}}