ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR786/003/SRR7868103/SRR7868103.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR786/002/SRR7868102/SRR7868102.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR786/001/SRR7868101/SRR7868101.fastq.gzprimary200OKGenomicsCase Western Reserve Universityhttps://www.ebi.ac.uk/ena/browser/view/PRJNA491650Mus musculusThe RNA binding protein Dazl is essential for gametogenesis, but its direct in vivo functions, RNA targets, and the molecular basis for germ cell loss in DAZL null mice are unknown. Here, we mapped transcriptome-wide Dazl-RNA interactions in vivo, revealing Dazl binding to thousands of mRNAs via polyA-proximal 3’UTR interactions. In parallel, fluorescence activated cell sorting and RNA-Seq identified mRNAs sensitive to Dazl deletion in male germ cells. Despite binding a broad set of mRNAs, integrative analyses indicate that Dazl post-transcriptionally controls only a subset of its mRNA targets, namely those corresponding to a network of genes critical for germ cell proliferation and survival. Additionally, we provide evidence that polyA sequences have key roles in specifying Dazl-RNA interactions across the transcriptome. Altogether, our results reveal a mechanism for Dazl-RNA binding, and illustrate that Dazl functions as a master regulator of a post-transcriptional mRNA program essential for germ cell survival. Overall design: iCLIP libraries from monoclonal cell line with doxycyline inducible Dazl expression (GC-1-spg parental line)xref:PubMed:30380414ENAOLM4, DNA polalpha, GIANT CHLOROPLAST 1, dazl1, Daz-like, ssq, pol alpha, Gm296, iCLIP., Viabilities, DNA pol-73, familial spastic paraparesis, polalpha, CG5923, DNApola, CG11754, Myo, FSP, polalpha50, DmelCG7108, Messenger, UNQ362, Pol, Survival, dnapol alpha 50, mat3(6), AAB24152, CG6349, NSX, GW112, hGC-1, MLRC, CG3595, Non Polyadenylated, Viability, E(mus304), individual-nucleotide resolution cross-linking and immunoprecipitation, dazh, RLC, p180, Polalpha 73kDa, Myo-II, DNApol-alpha, Polyadenylated Messenger, Gm913, dazl-B, messenger RNA, DmPolalpha, Polalpha, spgyla, Dazla, CG14530, swi7, template RNA, pPD4, POlalpha 180kDa, bA209J19.1, DmMRLC_C, DNApolalpha50, Dazl1, Dm Dock4, DAZLA, NMM, Tpx2, DAZH, iCLIP assay, ATGC1, Myo II, dazla, hOLfD, OlfD, DAZL1, pola, DmelCG3595, EIEE3, RGD1307826, DNApolalpha, RNA, DmelCG6349, DNApol, guanylyl cyclase 1, MLC, DNA Pol-alpha180, K18J17.8, Polyadenylated, CG7108, POLA, AW321876, Messenger RNA, sqh/RLC, sgh, myoII, Cell, Dmpol alpha, polA, Cell Viabilities, DmelCG5923, Dazh, spg, Sqh, Poly(A)+ mRNA, polalphap180, PRI1, INSDC_feature:mRNA, ARABIDOPSIS GUANYLYL CYCLASE 1, Gc1, Polyadenylated RNA, Squh, Squ, dazl-a, ATSULA, anon-WO0140519.257, Xdazl, Polyadenylated Messenger RNA, 183 kDa pol alpha, Poly(A)+ RNA, Polalpha 50kDa, Non Polyadenylated mRNA, protein_coding_transcript, mRNA, Poly(A) Tail, DNApol-alpha72, Non-Polyadenylated, GC1, RMLC, Tpx-2, NET44, squ, Dpri50, mat(3)6, Non-Polyadenylated mRNA, Pol alpha, DNApolalpha180, SPGYLA, DmelCG31048, Pola, 1300006L01Rik, DNA-Polalpha, K18J17_8, MyoII, MRLC, Polyadenylated mRNA, AI060884, Cell Viability, Poly(A) RNA, CG31048OLM4, DNA polalpha, GIANT CHLOROPLAST 1, dazl1, Daz-like, ssq, pol alpha, Gm296, iCLIP., Viabilities, DNA pol-73, familial spastic paraparesis, polalpha, CG5923, DNApola, CG11754, Myo, FSP, polalpha50, DmelCG7108, Messenger, UNQ362, Pol, Survival, dnapol alpha 50, mat3(6), AAB24152, CG6349, NSX, GW112, hGC-1, MLRC, CG3595, Non Polyadenylated, Viability, E(mus304), individual-nucleotide resolution cross-linking and immunoprecipitation, dazh, RLC, p180, Polalpha 73kDa, Myo-II, DNApol-alpha, Polyadenylated Messenger, Gm913, dazl-B, messenger RNA, DmPolalpha, Polalpha, spgyla, Dazla, CG14530, swi7, template RNA, pPD4, POlalpha 180kDa, bA209J19.1, DmMRLC_C, DNApolalpha50, Dazl1, Dm Dock4, DAZLA, NMM, Tpx2, DAZH, iCLIP assay, ATGC1, Myo II, dazla, hOLfD, OlfD, DAZL1, pola, DmelCG3595, EIEE3, RGD1307826, DNApolalpha, RNA, DmelCG6349, DNApol, guanylyl cyclase 1, MLC, DNA Pol-alpha180, K18J17.8, Polyadenylated, CG7108, POLA, AW321876, Messenger RNA, sqh/RLC, sgh, myoII, Cell, Dmpol alpha, polA, Cell Viabilities, DmelCG5923, Dazh, spg, Sqh, Poly(A)+ mRNA, polalphap180, PRI1, INSDC_feature:mRNA, ARABIDOPSIS GUANYLYL CYCLASE 1, Gc1, Polyadenylated RNA, Squh, Squ, dazl-a, ATSULA, anon-WO0140519.257, Xdazl, Polyadenylated Messenger RNA, 183 kDa pol alpha, Poly(A)+ RNA, Polalpha 50kDa, Non Polyadenylated mRNA, protein_coding_transcript, mRNA, Poly(A) Tail, DNApol-alpha72, Non-Polyadenylated, GC1, RMLC, Tpx-2, NET44, squ, Dpri50, mat(3)6, Non-Polyadenylated mRNA, Pol alpha, DNApolalpha180, SPGYLA, DmelCG31048, Pola, 1300006L01Rik, DNA-Polalpha, K18J17_8, MyoII, MRLC, Polyadenylated mRNA, AI060884, Cell Viability, Poly(A) RNA, CG31048falseDazl regulates germ cell survival through a network of polA-proximal mRNA interactions [GC1-spg cells (parental line) iCLIP]Dazl regulates germ cell survival through a network of polA-proximal mRNA interactions [GC1-spg cells (parental line) iCLIP]2025-09-242018-09-21PRJNA491650GSE1200981009030380414