ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR814/007/SRR8144737/SRR8144737.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR814/000/SRR8144730/SRR8144730.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR814/005/SRR8144735/SRR8144735.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR814/004/SRR8144734/SRR8144734.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR814/001/SRR8144731/SRR8144731.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR814/003/SRR8144733/SRR8144733.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR814/006/SRR8144736/SRR8144736.fastq.gzprimaryOK200GenomicsPascale Romby lab, UPR9002 - IBMC, CNRShttps://www.ebi.ac.uk/ena/browser/view/PRJNA503392Staphylococcus aureusThe virulon of Staphyloccocus aureus is controlled by intricate connections between transcriptional and post-transcriptional regulators including proteins and small non coding RNAs (sRNAs). Most of the sRNAs regulate gene expression through base-pairings with mRNAs. Here, we have applied and adapted the MS2-affinity purification approach coupled to RNA sequencing (MAPS) to determine the targetome of RsaI sRNA of S. aureus. Overall design: Affinity purification of in vivo regulatory complexes coupled with high throughput RNA sequencing methodology or MAPS standing for MS2 affinity purification coupled to RNApathogen:prioritypathogen:bacteriumpathogenbacteriaENAfalseMS2-affinity purification coupled with RNA sequencing (MAPS) reveals S. aureus RsaI sRNA targetomeMS2-affinity purification coupled with RNA sequencing (MAPS) reveals S. aureus RsaI sRNA targetome2025-09-242018-11-03PRJNA503392GSE1220651280