{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR818/005/SRR8180995/SRR8180995.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR818/006/SRR8180996/SRR8180996.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR818/004/SRR8180994/SRR8180994.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR818/002/SRR8181002/SRR8181002.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR818/009/SRR8180999/SRR8180999.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR818/007/SRR8180997/SRR8180997.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR818/001/SRR8181001/SRR8181001.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR818/008/SRR8180998/SRR8180998.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR818/000/SRR8181000/SRR8181000.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR818/003/SRR8180993/SRR8180993.fastq.gz"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Chemistry and Molecular Biology, University of Gothenburg"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA505169"],"scientific_name":["Saccharomyces cerevisiae"],"long_description":["Strong cellular stress causes wide-spread perturbations in the transcriptome and in several types of DNA/RNA interactions, and through incompletely understood pathways to formation of cytoplasmic stress granules (SGs). We have investigated the relationships between strong transcriptional induction, RNA:DNA hybrid stretches (R-loops), and SG formation under severe hyperosmotic and glucose stress. Several mutations affecting DNA processing proteins, including the DNA polymerase subunit Pol32, confer SG formation defects. Severe stress increased R loop levels globally. We found that facilitating removal of R-loops by RNase H overexpression, with activity towards RNA:DNA hybrids, accelerated and enhanced transcriptional induction of stress-activated genes. Thus, overexpression of RNase H1, but not RNase H2, reduced R-loops globally around the 1 h mark. Remarkably, it also reduced SG formation. We performed a genome-wide analysis of the induction or repression kinetics of gene expression under severe stress conditions. RNase H1 overexpression reduced R-loops locally in highly transcribed stress-affected genes, as expected. Notably, it also increased expression of several stress-induced genes. Conversely, in cells where R-loops are not efficiently resolved, transcriptional induction of the same genes under stress was muted and occurred with a delay. Thus, in pol32∆ mutants, where SG accumulation is delayed, R-loop levels are elevated, and induction of stress genes suppressed. The pol32∆ mutants are also refractory to the effects of RNase H1 overexpression on stress gene induction, R-loop resolution, and SG formation, indicating that Pol32 may act downstream of Rnh1 in the regulation of these three processes. These findings demonstrate an unexpected link between R-loops and formation of SGs. Together, these observations indicate that under stress, strong transcriptional induction of specific genes or genomic regions causes R-loop accumulation, which then requires RNase H1 activity for resolution. If unresolved, the accumulated R-loops impede continued stress-induced transcription, and delay or prevent SG formation. Overall design: Samples were collected every 30 min after onset of 1.5 M KCl stress, up to 2 h. Samples were collected from two independent replicate experiment time series Total number of samples = 10. Control samples were collected at the 0 h time point."],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"The DNA polymerase subunit Pol32 and RNase H1 are required for stress granule formation through R-loop regulation","description":"The DNA polymerase subunit Pol32 and RNase H1 are required for stress granule formation through R-loop regulation","dates":{"last_updated":"2025-09-24","first_public":"2022-01-01"},"accession":"PRJNA505169","cross_references":{"GEO":["GSE122423"],"taxon":["4932"]}}