ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR831/007/SRR8313017/SRR8313017_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR831/006/SRR8313016/SRR8313016_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR831/007/SRR8313007/SRR8313007_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR831/000/SRR8313010/SRR8313010_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR831/006/SRR8313006/SRR8313006_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR831/004/SRR8313014/SRR8313014_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR831/003/SRR8313013/SRR8313013_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR831/007/SRR8313017/SRR8313017_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR831/005/SRR8313015/SRR8313015_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR831/006/SRR8313016/SRR8313016_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR831/008/SRR8313008/SRR8313008_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR831/000/SRR8313010/SRR8313010_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR831/004/SRR8313014/SRR8313014_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR831/006/SRR8313006/SRR8313006_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR831/007/SRR8313007/SRR8313007_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR831/003/SRR8313013/SRR8313013_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR831/001/SRR8313011/SRR8313011_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR831/005/SRR8313015/SRR8313015_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR831/002/SRR8313012/SRR8313012_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR831/009/SRR8313009/SRR8313009_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR831/001/SRR8313011/SRR8313011_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR831/002/SRR8313012/SRR8313012_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR831/008/SRR8313008/SRR8313008_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR831/009/SRR8313009/SRR8313009_2.fastq.gzprimary200OKGenomicsBioinformatics Unit, Structural Biology, CNIOhttps://www.ebi.ac.uk/ena/browser/view/PRJNA509567Homo sapiensxref:PubMed:31403184We explored alternative splicing events upon hsa-miR-139-5p/HNRNPF axis regulation in our cell system using the Multivariate Analysis of Transcript Splicing (rMATS) computational tool (https://doi.org/10.1073/pnas.1419161111). Differential analysis of major splicing outcomes (skipped exon, alternative 5’/3’ splice, mutually exclusive exons and retained intron) revealed a total of 174 events significantly activated or repressed (FDR<0.05) upon HNRNPF regulation. Overall design: CAL62 isogenic cell lines expressing either mature hsa-miR-139-5p or a non-targeting control under a doxycycline inducible promoter were established. Cells were growth with or without doxycycline for 96 hours. The experiment was replicated 3 times and miRNA expression and HNRNPF mRNA/protein abundance quantified. HNRNPF was consistently decreases in doxycycline-treated hsa-miR-139-5p inducible cells.ENA9430057C20Rik, HH, Carcinoma, l(3)hh, Materials, R13-Ag, Thyroid Adenomas, Myosin regulatory light chain interacting protein, Adenomas, axis, 6.3.2.-, Neoplasms, bar, nectadrin, Gene, M1/69-J11D heat stable antigen, stalk, Hsa, HSA, HNRPF, Thyroid Carcinoma, IDOL, Cistrons, Cd24, cancer of the thyroid, CG4637, Thyroid, Hh, CG12249, AW228700, cancer of thyroid, anon-WO0134654.19, small cell lung carcinoma cluster 4 antigen, DmelCG4637, Hnrpf, bar3, Neoplasm, Genetic Materials, Cell., Oxidase assembly 1-like protein, DmelCG12249, thyroid, OK|SW-cl.23, Mir, MIR, Genetic Material, Thyroid Neoplasm, Miranda, Carcinomas, OK/SW-cl.23, lymphocyte antigen 52, l(3)neo56, l(3)neo57, MONDOA, Genetic, thyroid cancer, Adenoma, Mrt, Mira, C2, Inducible degrader of the LDL-receptor, INSDC_feature:gene, neoplasm of thyroid gland, mir, Cancers, Ly-52, Thyroid Adenoma, culm, Thyroid Cancers, OXA1Hs, OXA1-like protein, head and neck cancer, X62 heat stable antigen, AA407306, Myosin regulatory light chain-interacting protein, Thyroid Carcinomas, anon-WO0182946.19, Material, bar-3, MIRA, CD24A, Cistron, ly-52, bHLHe36, Thyroid Cancer, Cancer of the Thyroid, Idol, mcs94-1, thyroid gland neoplasm, Cancer of Thyroid, 4833420I20Rik, CD24, hg, Cancer, thyroid gland cancer9430057C20Rik, HH, Carcinoma, l(3)hh, Materials, R13-Ag, Thyroid Adenomas, Myosin regulatory light chain interacting protein, Adenomas, axis, 6.3.2.-, Neoplasms, bar, nectadrin, Gene, M1/69-J11D heat stable antigen, stalk, Hsa, HSA, HNRPF, Thyroid Carcinoma, IDOL, Cistrons, Cd24, cancer of the thyroid, CG4637, Thyroid, Hh, CG12249, AW228700, cancer of thyroid, anon-WO0134654.19, small cell lung carcinoma cluster 4 antigen, DmelCG4637, Hnrpf, bar3, Neoplasm, Genetic Materials, Cell., Oxidase assembly 1-like protein, DmelCG12249, thyroid, OK|SW-cl.23, Mir, MIR, Genetic Material, Thyroid Neoplasm, Miranda, Carcinomas, OK/SW-cl.23, lymphocyte antigen 52, l(3)neo56, l(3)neo57, MONDOA, Genetic, thyroid cancer, Adenoma, Mrt, Mira, C2, Inducible degrader of the LDL-receptor, INSDC_feature:gene, neoplasm of thyroid gland, mir, Cancers, Ly-52, Thyroid Adenoma, culm, Thyroid Cancers, OXA1Hs, OXA1-like protein, head and neck cancer, X62 heat stable antigen, AA407306, Myosin regulatory light chain-interacting protein, Thyroid Carcinomas, anon-WO0182946.19, Material, bar-3, MIRA, CD24A, Cistron, ly-52, bHLHe36, Thyroid Cancer, Cancer of the Thyroid, Idol, mcs94-1, thyroid gland neoplasm, Cancer of Thyroid, 4833420I20Rik, CD24, hg, Cancer, thyroid gland cancerfalseHsa-miR-139-5p/HNRNPF axis modulates gene-transcripts balance in thyroid cancer cellsHsa-miR-139-5p/HNRNPF axis modulates gene-transcripts balance in thyroid cancer cells2025-09-242019-08-28PRJNA509567GSE123680960631403184