{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR855/005/SRR8558635/SRR8558635.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR855/002/SRR8558632/SRR8558632.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR855/004/SRR8558634/SRR8558634.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR855/003/SRR8558633/SRR8558633.fastq.gz"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["AG Dobreva, Anatomy and Developmental Biology, Medical Faculty Mannheim/University of Heidelberg"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA521774"],"scientific_name":["Mus musculus"],"tag":["xref:PubMed:31024170"],"long_description":["Generation of widely differing and specialized cell types from a single totipotent zygote involves large-scale transcriptional changes and chromatin reorganization. Pioneer transcription factors play key roles in programming the epigenome and facilitating recruitment of additional regulatory factors during successive cell lineage specification and differentiation steps. Here we show that Isl1 acts as a pioneer factor driving cardiomyocyte lineage commitment by shaping the chromatin landscape of cardiac progenitor cells. Using an Isl1 hypomorphic mouse line which shows congenital heart defects, genome-wide profiling of Isl1 binding together with RNA- and ATAC-sequencing of cardiac progenitor cells and their derivatives, we uncover a regulatory network downstream of Isl1 that orchestrates cardiogenesis. Mechanistically, we show that Isl1 binds to compacted chromatin and woks in concert with the Brg1-Baf60c-based SWI/SNF complex to promote permissive cardiac lineage-specific alterations in the chromatin landscape not only of genes with critical functions in cardiac progenitor cells, but also of cardiomyocyte structural genes that are highly expressed when Isl1 itself is no longer present. Thus, the Isl1/Brg1-Baf60c complex plays a crucial role in orchestrating proper cardiogenesis and in establishing epigenetic memory of cardiomyocyte fate commitment. Overall design: Nkx2.5GFP reporter ES cell line stably transfected with Isl1Sh constructs were generated. Isl1Knockdown ES cell lines were differentiated into cardiovascular progenitors. Genomewide RNA-seq profiles were generated."],"repository":["ENA"],"name_synonyms":["Heart, Heart Muscle Cells, Ribonucleic, d230, Myocyte, islet1, dTAFII250, EfW1, prevention, Cardiac Muscle, dmTAF[[II]]230, Cardiac, dmTAF1, Taf230, ISL1, cardiac muscle fiber, Gene Products, cardiomyocyte, prevention and control, Muscle Cell, Non Polyadenylated, TAF250, Taf200, dTAF[[II]]250, reference sample, TFIID TAF250, cel, cell, FATE, Cardiac Myocytes, Taf1p, Muscle Cells, Epigenomic, RNA Gene Products., Non-Polyadenylated RNA, preventive measures, dTAF250, Xisl-1, Cardiomyocytes, Cardiac Myocyte, TAF, isl-1-eq-homeobox, Controlled, Controlling, RNA, dTAF[[II]]230, TAF[[II]]250, ISLET1, preventive therapy, Xislet-1, ribose nucleic acid, ribonucleic acids, Heart Muscle, TAF200, l(3)84Ab, RNS, function, BG:DS00004.13, TAFII-250, TAF250/230, cardiocyte, Cell, dTAF230, TAFII250, p230, yeast nucleic acid, cardiac muscle cell, Ribonukleinsaeure, CT43, TAF[[II]]250/230, TFIID, pentosenucleic acids, Ribonucleic acids, Cardiomyocyte, Isl-1, Taf[[II]]250, ribonucleic acid, Acid, TAF[[II]]230, Epigenetic, prophylaxis, Non Polyadenylated RNA, Non-Polyadenylated, TAF[II]250, Cardiac Muscle Cells, Ribonucleic Acid, islet-1, CG17603, TAF[[II]], Cardiac Muscle Cell, Heart Muscle Cell, DmelCG17603, Taf250, control, SR3-5, cardiac myocyte, Cells, heart muscle cell, Epigenetics, TAF230, TAF1"],"description_synonyms":["Heart, Heart Muscle Cells, Ribonucleic, d230, Myocyte, islet1, dTAFII250, EfW1, prevention, Cardiac Muscle, dmTAF[[II]]230, Cardiac, dmTAF1, Taf230, ISL1, cardiac muscle fiber, Gene Products, cardiomyocyte, prevention and control, Muscle Cell, Non Polyadenylated, TAF250, Taf200, dTAF[[II]]250, reference sample, TFIID TAF250, cel, cell, FATE, Cardiac Myocytes, Taf1p, Muscle Cells, Epigenomic, RNA Gene Products., Non-Polyadenylated RNA, preventive measures, dTAF250, Xisl-1, Cardiomyocytes, Cardiac Myocyte, TAF, isl-1-eq-homeobox, Controlled, Controlling, RNA, dTAF[[II]]230, TAF[[II]]250, ISLET1, preventive therapy, Xislet-1, ribose nucleic acid, ribonucleic acids, Heart Muscle, TAF200, l(3)84Ab, RNS, function, BG:DS00004.13, TAFII-250, TAF250/230, cardiocyte, Cell, dTAF230, TAFII250, p230, yeast nucleic acid, cardiac muscle cell, Ribonukleinsaeure, CT43, TAF[[II]]250/230, TFIID, pentosenucleic acids, Ribonucleic acids, Cardiomyocyte, Isl-1, Taf[[II]]250, ribonucleic acid, Acid, TAF[[II]]230, Epigenetic, prophylaxis, Non Polyadenylated RNA, Non-Polyadenylated, TAF[II]250, Cardiac Muscle Cells, Ribonucleic Acid, islet-1, CG17603, TAF[[II]], Cardiac Muscle Cell, Heart Muscle Cell, DmelCG17603, Taf250, control, SR3-5, cardiac myocyte, Cells, heart muscle cell, Epigenetics, TAF230, TAF1"],"additional_accession":[]},"is_claimable":false,"name":"Pioneering function of Isl1 in the epigenetic control of cardiomyocyte cell fate [Isl1_RNA_seq]","description":"Pioneering function of Isl1 in the epigenetic control of cardiomyocyte cell fate [Isl1_RNA_seq]","dates":{"last_updated":"2025-09-24","first_public":"2019-05-04"},"accession":"PRJNA521774","cross_references":{"GEO":["GSE126401"],"taxon":["10090"],"PubMed":["31024170"]}}