{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR863/007/SRR8634167/SRR8634167.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR863/008/SRR8634168/SRR8634168.fastq.gz"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["State Key Laboratory of Virology, College of Life Sciences, Wuhan University"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA524341"],"scientific_name":["Drosophila melanogaster"],"tag":["xref:PubMed:30837153"],"long_description":["Drosophila Trf4-1 is a polyadenylation polymerase or terminal nucleotidyl transferase (PAP/TENT) that has been reported to add poly(A) tails to snRNAs in nucleus or mRNAs in cytoplasm. Here, we found that the loss of Trf4-1 resulted in the reduction of mRNAs and primary miRNAs (pri-miRNAs) in both Drosophila S2 cells and adult flies. Interestingly, the role of Trf4-1 in transcription is independent of its PAP/TENT activity. Moreover, using the chromatin immunoprecipitation assay, we uncovered that the loss of Trf4-1 led to abnormal RNA polymerase II accumulation and reduced H3K4me3 binding in promoter regions. Overall design: Small RNA profile of RNAi based knock-down cells"],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"Drosophila Trf4-1 involvement in mRNA and primary miRNA transcription","description":"Drosophila Trf4-1 involvement in mRNA and primary miRNA transcription","dates":{"last_updated":"2025-09-24","first_public":"2019-05-02"},"accession":"PRJNA524341","cross_references":{"GEO":["GSE127210"],"taxon":["7227"],"PubMed":["30837153"]}}