ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR863/007/SRR8634167/SRR8634167.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR863/008/SRR8634168/SRR8634168.fastq.gzprimaryOK200GenomicsState Key Laboratory of Virology, College of Life Sciences, Wuhan Universityhttps://www.ebi.ac.uk/ena/browser/view/PRJNA524341Drosophila melanogasterxref:PubMed:30837153Drosophila Trf4-1 is a polyadenylation polymerase or terminal nucleotidyl transferase (PAP/TENT) that has been reported to add poly(A) tails to snRNAs in nucleus or mRNAs in cytoplasm. Here, we found that the loss of Trf4-1 resulted in the reduction of mRNAs and primary miRNAs (pri-miRNAs) in both Drosophila S2 cells and adult flies. Interestingly, the role of Trf4-1 in transcription is independent of its PAP/TENT activity. Moreover, using the chromatin immunoprecipitation assay, we uncovered that the loss of Trf4-1 led to abnormal RNA polymerase II accumulation and reduced H3K4me3 binding in promoter regions. Overall design: Small RNA profile of RNAi based knock-down cellsENAfalseDrosophila Trf4-1 involvement in mRNA and primary miRNA transcriptionDrosophila Trf4-1 involvement in mRNA and primary miRNA transcription2025-09-242019-05-02PRJNA524341GSE127210722730837153