ENAGenomicsMedical University Viennahttps://www.ebi.ac.uk/ena/browser/view/PRJNA525984Homo sapiensxref:PubMed:31570701The secretome of y-irradiated peripheral blood mononuclear cells (PBMC) is well known for its pleiotropic effects on tissue protection and regeneration. Irradiation of PBMC lead to necroptosis of a certain amount of PBMC in a cell-sorter analysis. The aim of this study was to investiage the trancriptomic changes of PBMC and PBMC subsets (CD4, CD8, CD19, CD56, CD14) in response to y-irradiation Overall design: In total 72 samples obtained from four healthy donors were analysed (0h= cells immediately after purification, 24h_0Gy = cells cultivated for 24 hours without prior radiation 24h_60Gy = cell cultivated for 24 hours after irradiation with 60Gy). At each time point six different cell populations were analysed (unseparated PBMC, and separated PBMC subsets: CD4, CD8, CD14 CD19 CD56).ENAPeripheral Blood Human Mononuclear Cells, Peripheral Blood Mononuclear Cell, Mononuclear Leukocyte., Leukocyte, Mononuclear, function, Mononuclear Leukocytes, PBMC Peripheral Blood Mononuclear Cells, Peripheral Blood Mononuclear CellsPeripheral Blood Human Mononuclear Cells, Peripheral Blood Mononuclear Cell, Mononuclear Leukocyte., Leukocyte, Mononuclear, function, Mononuclear Leukocytes, PBMC Peripheral Blood Mononuclear Cells, Peripheral Blood Mononuclear CellsfalseChanges in the biologica function of necroptotic, peripheral blood mononuclear cells under irradiation in vitroChanges in the biologica function of necroptotic, peripheral blood mononuclear cells under irradiation in vitro2025-09-242019-10-10PRJNA525984GSE127982960631570701