ENAGenomicsYan Lab, Pathology, Duke Universityhttps://www.ebi.ac.uk/ena/browser/view/PRJNA526085Homo sapiensxref:PubMed:31292202Here, we describe and characterize CRISPR-Cas9 based isogenic cell line models using patient-derived IDH1R132H/WT glioma cell lines, and we use these models to explore changes in DNA methylation following genetic deletion of IDH1 alleles that ablate D2HG production. Overall design: Comparison of CRISPR IDH1 targeted vs. parental cells or unedited clones methylation values.ENAGlioma 1, Glioma NOS, Glioma (except Nasal glioma, wide/broad, cou, GLM, glial tumors, malignant, Oxidative Demethylations, neuroglial tumor, Demethylation, obsolete_glioma, Mixed Glioma, Malignant Glioma, Neuroglial Tumor, broad, tumour of the neuroglia, Tumor, Tl3, Tl2, Malignant, Client, neoplasm of neuroglia, Lr, Demethylations, [M]Glioma NOS (morphologic abnormality), Oxidative, tumor of the neuroglia, [M]Gliomas (morphologic abnormality), Cell., Low, Gliomas, Mixed, Glial Cell Tumor, [M]Glioma NOS, Mixed Gliomas, Glial Tumor, NOS (except Nasal glioma, Oxidative Demethylation, me75, Neuroglial Neoplasm, glioma (morphologic abnormality), Genomes, Neuroglial Neoplasms, Glioma NOS (morphologic abnormality), neuroglial tumour, demethylation, Glial Cell, Malignant glioma, Neoplasm of the Neuroglia, patient, Tumor of Neuroglia, whole genome, Glial Neoplasm, D17Mit170, T1, neuroglial neoplasm, loss of, tumor of neuroglia, Neoplasm of Neuroglia, wide, glial tumor, no ICD-O subtype, Patient, Tumor of the Neuroglia, Clients, tumour of neuroglia, glial tumour, Glial Cell Tumors, Bra, not neoplastic), Gliomas (morphologic abnormality), Malignant Gliomas, Glioma, glial neoplasm, [M]Gliomas, malignant (morphologic abnormality), glioma, Tumors of Neuroglia, neoplasm of the neuroglia, Neoplasms of Neuroglia, TumorsGlioma 1, Glioma NOS, Glioma (except Nasal glioma, wide/broad, cou, GLM, glial tumors, malignant, Oxidative Demethylations, neuroglial tumor, Demethylation, obsolete_glioma, Mixed Glioma, Malignant Glioma, Neuroglial Tumor, broad, tumour of the neuroglia, Tumor, Tl3, Tl2, Malignant, Client, neoplasm of neuroglia, Lr, Demethylations, [M]Glioma NOS (morphologic abnormality), Oxidative, tumor of the neuroglia, [M]Gliomas (morphologic abnormality), Cell., Low, Gliomas, Mixed, Glial Cell Tumor, [M]Glioma NOS, Mixed Gliomas, Glial Tumor, NOS (except Nasal glioma, Oxidative Demethylation, me75, Neuroglial Neoplasm, glioma (morphologic abnormality), Genomes, Neuroglial Neoplasms, Glioma NOS (morphologic abnormality), neuroglial tumour, demethylation, Glial Cell, Malignant glioma, Neoplasm of the Neuroglia, patient, Tumor of Neuroglia, whole genome, Glial Neoplasm, D17Mit170, T1, neuroglial neoplasm, loss of, tumor of neuroglia, Neoplasm of Neuroglia, wide, glial tumor, no ICD-O subtype, Patient, Tumor of the Neuroglia, Clients, tumour of neuroglia, glial tumour, Glial Cell Tumors, Bra, not neoplastic), Gliomas (morphologic abnormality), Malignant Gliomas, Glioma, glial neoplasm, [M]Gliomas, malignant (morphologic abnormality), glioma, Tumors of Neuroglia, neoplasm of the neuroglia, Neoplasms of Neuroglia, TumorsfalseLoss of IDH1R132H mediated D-2HG production induces genome-wide demethylation in GCIMP low loci in patient-derived glioma cellsLoss of IDH1R132H mediated D-2HG production induces genome-wide demethylation in GCIMP low loci in patient-derived glioma cells2025-09-242019-11-09PRJNA526085GSE128032960631292202