ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/005/SRR8992035/SRR8992035_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/009/SRR8992019/SRR8992019_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/004/SRR8992034/SRR8992034_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/003/SRR8992033/SRR8992033_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/001/SRR8992031/SRR8992031_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/002/SRR8992032/SRR8992032_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/000/SRR8992030/SRR8992030_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/006/SRR8992026/SRR8992026_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/005/SRR8992025/SRR8992025_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/001/SRR8992031/SRR8992031_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/001/SRR8992021/SRR8992021_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/002/SRR8992032/SRR8992032_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/000/SRR8992020/SRR8992020_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/007/SRR8992027/SRR8992027_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/008/SRR8992028/SRR8992028_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/008/SRR8992018/SRR8992018_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/000/SRR8992030/SRR8992030_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/009/SRR8992029/SRR8992029_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/007/SRR8992027/SRR8992027_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/009/SRR8992029/SRR8992029_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/005/SRR8992025/SRR8992025_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/004/SRR8992024/SRR8992024_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/008/SRR8992028/SRR8992028_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/005/SRR8992035/SRR8992035_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/003/SRR8992023/SRR8992023_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/003/SRR8992033/SRR8992033_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/004/SRR8992034/SRR8992034_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/002/SRR8992022/SRR8992022_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/004/SRR8992024/SRR8992024_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/002/SRR8992022/SRR8992022_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/003/SRR8992023/SRR8992023_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/008/SRR8992018/SRR8992018_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/009/SRR8992019/SRR8992019_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/000/SRR8992020/SRR8992020_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/006/SRR8992026/SRR8992026_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR899/001/SRR8992021/SRR8992021_1.fastq.gzprimaryOK200GenomicsUC Davishttps://www.ebi.ac.uk/ena/browser/view/PRJNA540393T cell cytokines contribute to immunity against Staphylococcus aureus, but the predominant T cell subsets involved are unclear. In a S. aureus skin infection mouse model, we found that the IL-17 response was mediated by γδ T cells, which trafficked from lymph nodes to the infected skin to induce neutrophil recruitment, proinflammatory cytokines IL-1α, IL-1β and TNF and host defense peptides. RNA-seq for TRG and TRD sequences in lymph nodes and skin revealed a single clonotypic expansion of the encoded complementarity-determining region 3 amino acid sequence, which could be generated by canonical nucleotide sequences of TRGV5 or TRGV6 and TRDV4. However, only TRGV6 and TRDV4 but not TRDV5 sequences expanded. Finally, Vγ6+ T cells were predominant γδ T cell subset that produced IL 17A as well as IL-22, TNF and IFNγ, indicating a broad and substantial role for clonal Vγ6+Vδ4+ T cells in immunity against S. aureus skin infections.ENAfalseClonal Vγ6+Vδ4+ T cells promote IL-17-mediated immunity against Staphylococcus aureus skin infection2023-05-172019-05-19PRJNA540393