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Universityhttps://www.ebi.ac.uk/ena/browser/view/PRJNA548234Homo sapiensRNA polymerase IIo ChIP-seq was performed to investigate the role of ubiquitination at the K1268 residue of human RPB1: the K1268R mutant lost transcription-coupled nucleotide excision repair (TC-NER) ability, resulting in induction of premature aging phenotypes. Wild type, the K1268R mutant, and TC-NER deficient HeLa cells were employed for the experiment. RPB1 molecules were immunoprecipitated with anti- phopho-Ser2-CTD (RPB1-Ser2: 3E10, Ab5095), or -Ser5-CTD (RPB1-Ser5: 3E8) antibodies, as well as with an anti-pan-RNA pol II (RPB1-pan: A304405A) antibody.ENAHITS-CLIP, High Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, RNA, Ribonucleic, ChIP-Chip, Chromatin Immuno-precipitation, ribose nucleic acid, Cross Linking and Immunoprecipitation Followed by Deep Sequencing, ribonucleic acids, ChIP Sequencing, CLIP-Seq, RNS, Assay for Transposase-Accessible Chromatin Using Sequencing, ChIP-PET, Cell, ChIP-Exo, HeLa Cell, yeast nucleic acid, Ribonukleinsaeure, Chromatin Immunoprecipitation Sequencing-Chip, Gene Products, pentosenucleic acids, Ribonucleic acids, High-Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, Non Polyadenylated, RNA Gene Products, ChIA-PET, ribonucleic acid, Acid, TCR, Chromatin Immunoprecipitation Sequencing Chip, Chromatin Immuno precipitation Sequencing, ChIP, Chromatin Immunoprecipitation Paired End Tag, Non Polyadenylated RNA, Chromatin Immuno Precipitation Paired End Tag, HeLa, Non-Polyadenylated, Cross-Linking and Immunoprecipitation Followed by Deep Sequencing, Chromatin Immunoprecipitation, Chromatin Immuno-precipitation Sequencing, Ribonucleic Acid, ChIP Exonuclease, HeLa., ChIP-Seq, Sequencing, Assay for Transposase Accessible Chromatin Using Sequencing, Non-Polyadenylated RNA, Chromatin Immunoprecipitation Paired-End Tag, Chromatin Immuno-Precipitation Paired-End Tag, Cells, ATAC-Seq, Chromatin Immunoprecipitation Sequencing-Chips, ChIP-ExonucleaseHuman, Modern., human being, Man (Taxonomy), Homo sapiens, man, Man, human, Modern ManfalseHomo sapiensRNA polymerase IIo ChIP-seq by employing the K1268R RPBI mutant and TC-NER deficient HeLa cells2025-09-242020-01-29PRJNA5482349606