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CaMKII is a widely expressed enzyme whose activation by oxidation of regulatory domain methionines (ox-CaMKII) contributes to cardiovascular disease, asthma, and cancer. Here we integrate comparative genomic and experimental data to show that CaMKII activation by ROS arose more than half-a-billion years ago on the vertebrate stem lineage where it constituted a bridge between ROS and increased intracellular Ca2+ release, exercise responsive gene transcription, and improved performance in skeletal muscle.  These enhancements to fight-or-flight physiology were likely key in facilitating a well-evidenced shift in the behavioural ecology of our immediate chordate ancestors, and, in turn, the evolutionary success of vertebrates. Still, the ox-CaMKII innovation must be considered a critical evolutionary trade-off, as it rendered us more susceptible to common and often fatal diseases by providing ROS an expanded role in those processes. This poly(A+) transcriptome profiling study examined the actue transcriptional response of mouse skeletal muscles three hours after a single bout of submaximal exericse in wildtype and mutant mice whose CaMKIIg cannot be activated by reactive oxygen species. Overall design: Wildtype and CaMK2gVV/CaMK2gVV mice were either kept sedentary or forced to performed a submaximal running exercise. Four mice per genotype were used for each condition. Three hours after the group of exercised mice finished running, all mice were sacrifed for mRNA sequencing by Illumina NovaSeq 6000."],"repository":["ENA"],"description_synonyms":["3.4.22.-, polynuclear neutrophilic leukocyte, muscle system, RNA Sequence Determination, Activity, Transcriptome Profile, Laboratory, Sequence Determination, RNA Sequence, Mus domesticus, musculature system, Training, musculus quadriceps femoris, Isometric, Physical, Gene Expression Profile, muscles, Gene, mini-ICE, Muskelsystem, CASP-14, Exercise Training, Profiles, House Mouse, quadriceps muscle, neutrophilic leucocyte, House, Physical Exercises, neutrophil leukocyte, Mus musculus domesticus, Isometric., Analysis, Mice, Acute Exercises, Muscle Tissues, Aerobic Exercises, Analyses, Determination, Tissues, Swiss, Profile, Physical Activity, neutrophil leucocyte, Tissue, Swiss Mice, neutrophilic leukocyte, Sequence Determinations, Signatures, Sequencing, muscule system, RNA Sequence Analyses, Expression Signature, polymorphonuclear leukocyte, polynuclear neutrophilic leucocyte, Transcriptomes, RNA Sequencing, Exercises, Sequence Analyses, RNA, Transcriptome, quadriceps muscle of thigh, musculi, RNA Sequence Determinations, set of muscles, Expression Profiles, mouse, set of skeletal muscles, Exercise, Aerobic, Muscle, Determinations, Acute onset, whole transcriptome, Gene Expression, neutrocyte, Mus, Expression Signatures, Gene Expression Signatures, Mini-ICE, Acute Exercise, Gene Expression Signature, Isometric Exercises, Expression Profile, Physical Exercise, Transcriptome Profiles, Aerobic Exercise, Mus musculus, muscles set, Caspase-14 subunit p10, mice, motor system, Swiss Mouse, quadriceps, House Mice, Caspase-14 subunit p19, MICE, Trainings, Exercise Trainings, Laboratory Mice, domesticus, Isometric Exercise, Activities, quadricep muscle, Acute, Physical Activities, Gene Expression Profiles, RNA Sequence Analysis, PMN, polymorphonuclear leucocyte, musculature, quadriceps muscle of the thigh, Mouse, Signature, poly, Muscle Tissue, Laboratory Mouse, polymorphonuclear neutrophil, muscle group"],"name_synonyms":["3.4.22.-, polynuclear neutrophilic leukocyte, muscle system, RNA Sequence Determination, Activity, Transcriptome Profile, Laboratory, Sequence Determination, RNA Sequence, Mus domesticus, musculature system, Training, musculus quadriceps femoris, Isometric, Physical, Gene Expression Profile, muscles, Gene, mini-ICE, Muskelsystem, CASP-14, Exercise Training, Profiles, House Mouse, quadriceps muscle, neutrophilic leucocyte, House, Physical Exercises, neutrophil leukocyte, Mus musculus domesticus, Isometric., Analysis, Mice, Acute Exercises, Muscle Tissues, Aerobic Exercises, Analyses, Determination, Tissues, Swiss, Profile, Physical Activity, neutrophil leucocyte, Tissue, Swiss Mice, neutrophilic leukocyte, Sequence Determinations, Signatures, Sequencing, muscule system, RNA Sequence Analyses, Expression Signature, polymorphonuclear leukocyte, polynuclear neutrophilic leucocyte, Transcriptomes, RNA Sequencing, Exercises, Sequence Analyses, RNA, Transcriptome, quadriceps muscle of thigh, musculi, RNA Sequence Determinations, set of muscles, Expression Profiles, mouse, set of skeletal muscles, Exercise, Aerobic, Muscle, Determinations, Acute onset, whole transcriptome, Gene Expression, neutrocyte, Mus, Expression Signatures, Gene Expression Signatures, Mini-ICE, Acute Exercise, Gene Expression Signature, Isometric Exercises, Expression Profile, Physical Exercise, Transcriptome Profiles, Aerobic Exercise, Mus musculus, muscles set, Caspase-14 subunit p10, mice, motor system, Swiss Mouse, quadriceps, House Mice, Caspase-14 subunit p19, MICE, Trainings, Exercise Trainings, Laboratory Mice, domesticus, Isometric Exercise, Activities, quadricep muscle, Acute, Physical Activities, Gene Expression Profiles, RNA Sequence Analysis, PMN, polymorphonuclear leucocyte, musculature, quadriceps muscle of the thigh, Mouse, Signature, poly, Muscle Tissue, Laboratory Mouse, polymorphonuclear neutrophil, muscle group"],"additional_accession":[]},"is_claimable":false,"name":"RNA sequencing of poly(A+) transcriptome of quadriceps muscles from wildtype and CaMK2gVV/CaMK2gVV mice under sedentary and post-acute exercise conditions","description":"RNA sequencing of poly(A+) transcriptome of quadriceps muscles from wildtype and CaMK2gVV/CaMK2gVV mice under sedentary and post-acute exercise conditions","dates":{"last_updated":"2025-09-24","first_public":"2021-05-03"},"accession":"PRJNA548281","cross_references":{"GEO":["GSE132520"],"taxon":["10090"],"PubMed":["34039988"]}}