ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR930/007/SRR9300377/SRR9300377_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR930/000/SRR9300380/SRR9300380_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR930/008/SRR9300378/SRR9300378_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR930/009/SRR9300379/SRR9300379_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR930/008/SRR9300378/SRR9300378_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR930/000/SRR9300380/SRR9300380_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR930/007/SRR9300377/SRR9300377_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR930/009/SRR9300379/SRR9300379_1.fastq.gzprimaryOK200GenomicsNK65NYUNational Institute of Allergy and Infectious Diseaseshttps://www.ebi.ac.uk/ena/browser/view/PRJNA548804Plasmodium bergheipathogen:protozoanpathogenxref:EuropePMC:PMC7002124Antibodies play a central role in malaria immunity but their acquisition is both remarkably slow and unpredictable. At present we know little about the malaria parasite genesthat influence host immunity. Here we show that a single nucleotide polymorphism (SNP) in thegene encoding an ApiAP2 transcription factor in the rodent malaria parasite Plasmodium berghei(Pb) NK65, resulting in a single amino acid change (a S to F), converted a nonimmunogenicparasite (PbNK65S) to a parasite (PbNK65F) that induced protective immunity. The S to F SNPis unique and has occurred only once in the evolution of Plasmodium namely in the rodentparasite PbANKA. PbNK65F parasites differentially expressed 46 genes, most of which arepredicted to play roles in immune evasion. PbNK65F infections resulted in a large expansion ofgerminal center B cells, plasma cell lineage B cells and T follicular helper cells by a γ-interferondependentprocess resulting in higher levels of infected red blood cell-specific TH1-type IgG2band 2c antibodies. Thus, the Pb ApiAP2 transcription factor functions as a critical parasitevirulence factor in malaria infections.protozoaENARNA polymerase II core promoter proximal region sequence-specific binding, host organism, T-cell leukemia, Process, postnatal development, AGL4, Plasmodium, Plasmodium bergheus, zinc ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, homeobox 1, growth and development, Factor, SEPALLATA 2, metal ion regulated sequence-specific DNA binding RNA polymerase II transcription factor activity, Transcription Factor, transcription factor activity, development, berghei, zinc ion regulated core promoter proximal region sequence-specific DNA binding, RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity, RNA polymerase II transcription factor activity, sequence-specific distal enhancer binding RNA polymerase II transcription factor activity, Immune., metal ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, KNOTTED1-like homeobox gene 5, nucleotides, F14P3.4, Transcription, Immune Processes, Immune Responses, Factors, postnatal growth, F14P3_4, F10N7_150, metal ion regulated core promoter proximal region sequence-specific binding, copper ion regulated core promoter proximal region sequence-specific binding, RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, Transcription factor, F10N7.150, Immune Response, Immune, RNA polymerase II proximal promoter sequence-specific DNA binding, copper ion regulated proximal promoter sequence-specific DNA binding, copper ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, AGAMOUS-like 4, Response, zinc ion regulated proximal promoter sequence-specific DNA binding, sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity, Nucleotide, Immune Process, metal ion regulated sequence-specific DNA binding, growth, metal ion regulated proximal promoter sequence-specific DNA binding, TRANSCRIPTION FACTOR, RNA polymerase II distal enhancer sequence-specific bindingstrain, Sprain, Plasmodium, Strain., Plasmodium bergheus, cultivar, Strains, berghei, Sprains, Strains and Sprains, ecotypefalsePlasmodium berghei strain:NK65NYUA single nucleotide polymorphism in a Plasmodium berghei ApiAP2 transcription factor alters the development of host immunity2025-09-242020-02-13PRJNA5488045821