{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR992/005/SRR9925405/SRR9925405.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR992/004/SRR9925404/SRR9925404.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR992/001/SRR9925411/SRR9925411.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR992/007/SRR9925407/SRR9925407.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR992/000/SRR9925410/SRR9925410.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR992/008/SRR9925408/SRR9925408.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR992/009/SRR9925409/SRR9925409.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR992/006/SRR9925406/SRR9925406.fastq.gz"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["UW Madison"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA559267"],"scientific_name":["Homo sapiens"],"tag":["xref:PubMed:31551422"],"long_description":["Development of treatments for vocal dysphonia has been inhibited by lack of human vocal fold (VF) mucosa models because of difficulty in procuring VF epithelial cells, epithelial cells’ limited proliferative capacity and absence of cell lines. We report development of engineered VF mucosae from hiPSC, transfected via TALEN constructs for green fluorescent protein, that mimic development of VF epithelial cells in utero. Modulation of FGF signaling achieves stratified squamous epithelium from definitive and anterior foregut derived cultures. Robust culturing of these cells on collagen-fibroblast constructs produces three-dimensional models comparable to in vivo VF mucosa. Second, we demonstrate mucosal inflammation upon exposure of these constructs to 5% cigarette smoke extract. Upregulation of pro-inflammatory genes in epithelium and fibroblasts leads to aberrant VF mucosa remodeling. Collectively, our results demonstrate that hiPSC-derived VF mucosa is a versatile tool for future investigation of genetic and molecular mechanisms underlying epithelium-fibroblasts interactions in health and disease. Overall design: Comppared mRNA profiles of 4 samples from human primary vocal fold (VF) epithelial cell with 4 samples from human induced pluripotent stem cell derived vocal fold basal progenitors (iPScell-derived VBPs)"],"repository":["ENA"],"description_synonyms":["Fold, Ligament, Mucosa, human induced pluripotent stem cell, postnatal development., Fibroblast-Derived IPS Cell, Stimmband, single-organism developmental process, Induced Pluripotent Stem Cell, IPS, Muscularis Mucosae, Cords, vocal band, Fibroblast Derived IPS Cells, hiPSC, growth and development, vocal chord, IPS Cells, Cell, Cord, Muscularis, development, Mucosal, Fibroblast Derived Induced Pluripotent Stem Cells, mucosal region, Mucosae, Lamina Propria, Vocal, IPS Cell, Mucosal Tissue, Fibroblast-Derived IPS Cells, Membranes, Vocal Ligament, tunica mucosa, Tissues, Fibroblast-Derived IPS, organ mucosa, Tissue, postnatal growth, Vocal Fold, Mucous, Vocal Folds, vocal fold, Membrane, Fibroblast-Derived, region of mucosa, mucous membrane, Human Induced Pluripotent Stem Cells, Lamina, mucosa of organ, Mucous Membranes, Propria, Cells, Fibroblast-Derived Induced Pluripotent Stem Cells, mucosa of organ part, Stimmlippe, Human Induced Pluripotent Stem Cell, growth, Vocal Ligaments, Folds, plica vocalis, Ligaments, vocal cord, Mucosal Tissues, Vocal Cord"],"name_synonyms":["Fold, Ligament, Mucosa, human induced pluripotent stem cell, postnatal development., Fibroblast-Derived IPS Cell, Stimmband, single-organism developmental process, Induced Pluripotent Stem Cell, IPS, Muscularis Mucosae, Cords, vocal band, Fibroblast Derived IPS Cells, hiPSC, growth and development, vocal chord, IPS Cells, Cell, Cord, Muscularis, development, Mucosal, Fibroblast Derived Induced Pluripotent Stem Cells, mucosal region, Mucosae, Lamina Propria, Vocal, IPS Cell, Mucosal Tissue, Fibroblast-Derived IPS Cells, Membranes, Vocal Ligament, tunica mucosa, Tissues, Fibroblast-Derived IPS, organ mucosa, Tissue, postnatal growth, Vocal Fold, Mucous, Vocal Folds, vocal fold, Membrane, Fibroblast-Derived, region of mucosa, mucous membrane, Human Induced Pluripotent Stem Cells, Lamina, mucosa of organ, Mucous Membranes, Propria, Cells, Fibroblast-Derived Induced Pluripotent Stem Cells, mucosa of organ part, Stimmlippe, Human Induced Pluripotent Stem Cell, growth, Vocal Ligaments, Folds, plica vocalis, Ligaments, vocal cord, Mucosal Tissues, Vocal Cord"],"additional_accession":[]},"is_claimable":false,"name":"Human induced pluripotent stem cell-derived vocal fold mucosa mimics development and responses to smoke exposure","description":"Human induced pluripotent stem cell-derived vocal fold mucosa mimics development and responses to smoke exposure","dates":{"last_updated":"2025-09-24","first_public":"2019-08-10"},"accession":"PRJNA559267","cross_references":{"GEO":["GSE135548"],"taxon":["9606"],"PubMed":["31551422"]}}