ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR100/015/SRR10031515/SRR10031515_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR100/013/SRR10031513/SRR10031513_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR100/019/SRR10031519/SRR10031519_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR100/015/SRR10031515/SRR10031515_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR100/012/SRR10031512/SRR10031512_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR100/017/SRR10031517/SRR10031517_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR100/018/SRR10031518/SRR10031518_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR100/014/SRR10031514/SRR10031514_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR100/016/SRR10031516/SRR10031516_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR100/018/SRR10031518/SRR10031518_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR100/012/SRR10031512/SRR10031512_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR100/016/SRR10031516/SRR10031516_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR100/017/SRR10031517/SRR10031517_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR100/014/SRR10031514/SRR10031514_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR100/019/SRR10031519/SRR10031519_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR100/013/SRR10031513/SRR10031513_2.fastq.gzprimaryOK200GenomicsUniversity of Floridahttps://www.ebi.ac.uk/ena/browser/view/PRJNA562723Marine cyanobacteria are well-known as a rich source of structurally intriguing chemical entities to serve as therapeutic leads addressing unmet medical needs, especially in the field of fighting against cancer. Lagunamide D, a novel macrocyclic depsipeptide was identified from cyanobacteria with single-digit nanomolar antiproliferative activity against multiple cancer cell lines. The goal of this study was to explore the mechanism of action of this potent molecule to enable in-depth understanding of its roles in modulating cellular processes. RNA-sequencing analysis was applied to get an unbiased global view of how lagunamide D function in cells and reveal its actions on transcriptome scale at early (3 h) and late (12 h) time point.ENAstudy.falseMechanism of Action Study of Lagunamide D2023-05-192020-06-16PRJNA562723