{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR102/092/SRR10278792/SRR10278792.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR102/091/SRR10278791/SRR10278791.fastq.gz"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Peterson, Biomedical Engineering and Physiology, Mayo Clinic"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA577625"],"scientific_name":["Homo sapiens"],"tag":["xref:PubMed:32382023"],"long_description":["The generation of pancreatic cell types from renewable cell sources holds promise for cell replacement therapies for diabetes. Although most effort has focused on generating pancreatic beta cells, there is considerable evidence that glucagon secreting alpha cells are critically involved in disease progression and proper glucose control. Here we report on the generation of stem cell-derived human pancreatic alpha (SC-alpha) cells from pluripotent stem cells via a transient pre-alpha cell intermediate. These pre-alpha cells exhibit a transcriptional profile similar to mature alpha cells and although they produce proinsulin protein, they do not secrete significant amounts of processed insulin. The resulting SC-alpha cells do not express insulin, share an ultrastructure similar to cadaveric alpha cells, express and secrete glucagon in response to glucose and some glucagon secretagogues, and elevate blood glucose upon transplantation in mice. Overall design: Single cell mRNA sequencing data generated from stem cell-derived alpha cells and precursor (2 samples total, no replicates)"],"repository":["ENA"],"name_synonyms":["alpha-Ada, Colony Forming Unit, anon-EST:fe2E2, human being, BamF, alpha-Spectrin, l(3)alpha-Spec, Procedures, BamC, AP-2, 3A9, DmelCG1977, Modern, Progenitor Cells, Colony-Forming Units, alpha-Spect, Progenitor Cell, alpha-Spe, ham, Procedure, alpha-Spc, CG31654, Cell, l(2)SH2 0460, ada, Human, fs(3)neo61, CG10422, method, Techniques, alphaSpec, l(3)04276, alpha-ada, D-Ada, Colony-Forming Unit, Homo sapiens, Stem Cell, Method, method used in an experiment, klo, cg4260, Studies, Mother Cells, alpha-adaptin, MENE(2L)-A, Cell., alpha-Sp, dAP-2a, DmelCG10422, CG1977, alphaSp, spectrin, Technique, Man, Progenitor, alpha-spec, Mother Cell, l(2)06694, MENE (2L)-A, Man (Taxonomy), D-alphaAda, mating_type_alpha, CG4260, Stem, FCP-B, dre3, Mother, stem cell, Colony Forming Units, Methodological, alpha, AP-2alpha, Alpha-Adaptin, Methodological Study, man, human, alpha-spectrin, DmelCG4260, plan specification, Study, animal stem cell, AP2, Methodological Studies, l(3)62Bd, Bam-C, Modern Man, Cells, BAM, Bam, alpha mating type (yeast), l(2)SH0460, alfa-Spec, Spec, l(3)dre3"],"description_synonyms":["alpha-Ada, Colony Forming Unit, anon-EST:fe2E2, human being, BamF, alpha-Spectrin, l(3)alpha-Spec, Procedures, BamC, AP-2, 3A9, DmelCG1977, Modern, Progenitor Cells, Colony-Forming Units, alpha-Spect, Progenitor Cell, alpha-Spe, ham, Procedure, alpha-Spc, CG31654, Cell, l(2)SH2 0460, ada, Human, fs(3)neo61, CG10422, method, Techniques, alphaSpec, l(3)04276, alpha-ada, D-Ada, Colony-Forming Unit, Homo sapiens, Stem Cell, Method, method used in an experiment, klo, cg4260, Studies, Mother Cells, alpha-adaptin, MENE(2L)-A, Cell., alpha-Sp, dAP-2a, DmelCG10422, CG1977, alphaSp, spectrin, Technique, Man, Progenitor, alpha-spec, Mother Cell, l(2)06694, MENE (2L)-A, Man (Taxonomy), D-alphaAda, mating_type_alpha, CG4260, Stem, FCP-B, dre3, Mother, stem cell, Colony Forming Units, Methodological, alpha, AP-2alpha, Alpha-Adaptin, Methodological Study, man, human, alpha-spectrin, DmelCG4260, plan specification, Study, animal stem cell, AP2, Methodological Studies, l(3)62Bd, Bam-C, Modern Man, Cells, BAM, Bam, alpha mating type (yeast), l(2)SH0460, alfa-Spec, Spec, l(3)dre3"],"additional_accession":[]},"is_claimable":false,"name":"A method for the generation of human stem cell-derived alpha cells","description":"A method for the generation of human stem cell-derived alpha cells","dates":{"last_updated":"2025-09-24","first_public":"2019-10-17"},"accession":"PRJNA577625","cross_references":{"GEO":["GSE138857"],"taxon":["9606"],"PubMed":["32382023"]}}