ENAGenomicsInstitut Hospital del Mar d'Investigacions Mèdiqueshttps://www.ebi.ac.uk/ena/browser/view/PRJNA591645Homo sapiensImmunoglobulin A (IgA) is the most abundant antibody in the intestinal tract. Recent studies show that discrete subsets of gut human plasma cells (PCs) release IgA, which contributes to the maintenance of gut homeostasis. To better characterize the properties of these PC subsets, we performed global transcriptome analysis in naïve B cells as well as immature CD19+IgA+CD138- PCs, mature CD19+IgA+CD138+ PCs and late CD19-IgA+CD138+ PCs after their purification from human colon samples. Overall design: Control naive (N) CD45+CD10−CD19+IgA−IgM+IgD+ B cells as well as immature CD45+CD10−CD19+IgA+CD38+CD138− PCs (A), mature CD45+CD10−CD19+IgA+CD38+CD138+ PCs (B) and late CD45+CD10−CD19−IgA+CD38+CD138− PCs (D) were FACSorted from histologically normal tissue samples obtained from the ascending colon of four adult donors undergoing right hemicolectomy. The global transcriptome of each of these gut immune cell subsets was dissected by performing microarray analysis.ENAfalseTranscriptome analysis of human colonic IgA+ plasma cell subsets and naïve B cellsTranscriptome analysis of human colonic IgA+ plasma cell subsets and naïve B cells2025-09-242020-04-03PRJNA591645GSE1410059606