ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR105/067/SRR10582267/SRR10582267.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR105/073/SRR10582273/SRR10582273.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR105/081/SRR10582281/SRR10582281.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR105/064/SRR10582264/SRR10582264.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR105/068/SRR10582268/SRR10582268.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR105/070/SRR10582270/SRR10582270.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR105/079/SRR10582279/SRR10582279.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR105/075/SRR10582275/SRR10582275.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR105/077/SRR10582277/SRR10582277.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR105/066/SRR10582266/SRR10582266.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR105/069/SRR10582269/SRR10582269.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR105/072/SRR10582272/SRR10582272.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR105/074/SRR10582274/SRR10582274.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR105/078/SRR10582278/SRR10582278.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR105/071/SRR10582271/SRR10582271.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR105/082/SRR10582282/SRR10582282.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR105/080/SRR10582280/SRR10582280.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR105/065/SRR10582265/SRR10582265.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR105/063/SRR10582263/SRR10582263.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR105/076/SRR10582276/SRR10582276.fastq.gzprimaryOK200GenomicsPeter MacCallum Cancer Centrehttps://www.ebi.ac.uk/ena/browser/view/PRJNA593230Homo sapiensxref:PubMed:32917631We used 3' RNA-Seq to determine the effects of slow elongating Pol II on polyadenatltion site usages, also the effects of CDK12 and CDK13 inhibition caused by THZ531 on alternative polyadenylation Overall design: We performed 3’RNA-seq (Quantseq) on Flp-In-293 cells with slow elongating or wild-type a-amanitin resistant Pol II, as well as THZ531/DMSO treated HEK293T cells to compare the the effect of slow elongating Pol II to CDK12 and CDK13 inhibtion.ENAfalseQuant-seq analysis of differential polyadenylation site usages in caused by different Pol II mutants with slow elongation rates in Flp-In-293 cells and dual inhibition of CDK12 and CDK13 by THZ531 HEK293T cellsQuant-seq analysis of differential polyadenylation site usages in caused by different Pol II mutants with slow elongation rates in Flp-In-293 cells and dual inhibition of CDK12 and CDK13 by THZ531 HEK293T cells2025-09-242020-05-06PRJNA593230GSE141375960632917631