{"database":"ENA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Universidad Autonoma Metropolitana Unidad Iztapalapa"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA596004"],"scientific_name":["Homo sapiens"],"long_description":["Growth differentiation factor 11 (GDF11) has been characterized as a key regulator of differentiation in cells that retain stemness features. Recently, it has been reported that GDF11 exerts tumor suppressive properties in hepatocellular carcinoma cells decreasing clonogenicity, proliferation, spheroid formation and cellular function, all associated to a decrement in stemness features, resulting in mesenchymal to epithelial transition and loss of aggressiveness. The aim of the present work was to figure out the mechanism associated to the tumor suppressive properties displayed by GDF11 in liver cancer cells. Hepatocellular carcinoma-derived cell lines were exposed to GDF11 (50 ng/ml), RNA-seq analysis in Huh7 cell line revealed that GDF11 exerted profound transcriptomic impact involved regulation of cholesterol metabolic process, steroid metabolic process as well as key signaling pathways resembling endoplasmic reticulum-related functions. Cholesterol and triglycerides determination in Huh7 and Hep3B cells treated with GDF11 exhibited a significant decrement in the content of these lipids. The mTOR signaling pathway was downregulated, and this was associated to a decrement in key proteins involved in mevalonate pathway. In addition, real-time metabolism assessed by Seahorse technology showed abridged glycolysis as well as glycolytic capacity, closely related to an impaired oxygen consumption rate and decrement in ATP production. Finally, transmission electron microscopy revealed mitochondria abnormalities, such as cristae disarrangement, consistent with metabolic changes. Results provide evidence that GDF11 impairs cancer cell metabolism particularly targeting cholesterol homeostasis, glycolysis and mitochondria function and morphology."],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"Homo sapiens","description":"Huh-7 cell line treated with GDF-11","dates":{"last_updated":"2025-09-24","first_public":"2019-12-18"},"accession":"PRJNA596004","cross_references":{"taxon":["9606"]}}