ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/079/SRR10849979/SRR10849979_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/087/SRR10849987/SRR10849987_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/081/SRR10849981/SRR10849981_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/082/SRR10849982/SRR10849982_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/084/SRR10849984/SRR10849984_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/083/SRR10849983/SRR10849983_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/088/SRR10849988/SRR10849988_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/087/SRR10849987/SRR10849987_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/078/SRR10849978/SRR10849978_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/085/SRR10849985/SRR10849985_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/088/SRR10849988/SRR10849988_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/086/SRR10849986/SRR10849986_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/084/SRR10849984/SRR10849984_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/081/SRR10849981/SRR10849981_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/080/SRR10849980/SRR10849980_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/078/SRR10849978/SRR10849978_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/079/SRR10849979/SRR10849979_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/086/SRR10849986/SRR10849986_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/085/SRR10849985/SRR10849985_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/083/SRR10849983/SRR10849983_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/080/SRR10849980/SRR10849980_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/082/SRR10849982/SRR10849982_1.fastq.gzprimaryOK200GenomicsBenjamin Deneen, Cell and Gene Therapy, Baylor College of Medicinehttps://www.ebi.ac.uk/ena/browser/view/PRJNA599595Mus musculusThe goals of this study are to profile the molecular signatures of astrocytes from four brain regions (olfactory bulb, hippocampus, cortex, and brainstem) and determine if differential transcription factor enrichment may play a role in shaping astrocyte spatial diversity. We performed RNA-seq on astrocytes from the olfactory bulb, hippocampus, cortex, and brainstem, and determined region-specific molecular signatures. Using transcription factor motif discovery analysis on each region-specific gene signature we uncovered universal and region-specific transcription factor expression profiles. Overall design: Aldh1l1-GFP positive astrocytes were collected in triplicate from the olfactory bulb, hippocampus, and brainstem and duplicate from the cortex of 16-week old mice and profiled using RNA-seqxref:PubMed:32153350ENARNA polymerase II core promoter proximal region sequence-specific binding, T-cell leukemia, astrocytic glia, AGL4, Astroglia Cells, zinc ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, homeobox 1, Factor, SEPALLATA 2, metal ion regulated sequence-specific DNA binding RNA polymerase II transcription factor activity, Transcription Factor, Cell, transcription factor activity, zinc ion regulated core promoter proximal region sequence-specific DNA binding, Astroglial, Astrocyte, Astroglias, RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity, sequence, RNA polymerase II transcription factor activity, sequence-specific distal enhancer binding RNA polymerase II transcription factor activity, metal ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, KNOTTED1-like homeobox gene 5, region, Astroglial Cell, F14P3.4, Transcription, Factors, copper ion regulated core promoter proximal region sequence-specific binding., F14P3_4, F10N7_150, Astroglial Cells, metal ion regulated core promoter proximal region sequence-specific binding, RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, Transcription factor, primary structure of sequence macromolecule, F10N7.150, RNA polymerase II proximal promoter sequence-specific DNA binding, copper ion regulated proximal promoter sequence-specific DNA binding, heterogeneity, copper ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, AGAMOUS-like 4, site, Astroglia, zinc ion regulated proximal promoter sequence-specific DNA binding, sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity, metal ion regulated sequence-specific DNA binding, metal ion regulated proximal promoter sequence-specific DNA binding, TRANSCRIPTION FACTOR, Astroglia Cell, RNA polymerase II distal enhancer sequence-specific bindingRNA polymerase II core promoter proximal region sequence-specific binding, T-cell leukemia, astrocytic glia, AGL4, Astroglia Cells, zinc ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, homeobox 1, Factor, SEPALLATA 2, metal ion regulated sequence-specific DNA binding RNA polymerase II transcription factor activity, Transcription Factor, Cell, transcription factor activity, zinc ion regulated core promoter proximal region sequence-specific DNA binding, Astroglial, Astrocyte, Astroglias, RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity, sequence, RNA polymerase II transcription factor activity, sequence-specific distal enhancer binding RNA polymerase II transcription factor activity, metal ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, KNOTTED1-like homeobox gene 5, region, Astroglial Cell, F14P3.4, Transcription, Factors, copper ion regulated core promoter proximal region sequence-specific binding., F14P3_4, F10N7_150, Astroglial Cells, metal ion regulated core promoter proximal region sequence-specific binding, RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, Transcription factor, primary structure of sequence macromolecule, F10N7.150, RNA polymerase II proximal promoter sequence-specific DNA binding, copper ion regulated proximal promoter sequence-specific DNA binding, heterogeneity, copper ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, AGAMOUS-like 4, site, Astroglia, zinc ion regulated proximal promoter sequence-specific DNA binding, sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity, metal ion regulated sequence-specific DNA binding, metal ion regulated proximal promoter sequence-specific DNA binding, TRANSCRIPTION FACTOR, Astroglia Cell, RNA polymerase II distal enhancer sequence-specific bindingfalseProfiling astrocyte spatial heterogeneity and region-specific transcription factor networksProfiling astrocyte spatial heterogeneity and region-specific transcription factor networks2025-09-242020-01-15PRJNA599595GSE1432821009032153350