{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/051/SRR10850851/SRR10850851.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/052/SRR10850852/SRR10850852.fastq.gz"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Sysgem Genomics, Life Sciences, Ewha Womans University"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA599946"],"scientific_name":["Mus musculus"],"long_description":["Recently white adipose tissues (WAT) have been shown to be able to generate heat upon cold exposure through the expression of uncoupling protein-1 (UCP1), a molecule regulating mitochondrial proton gradient. Although the role of UCP1 has been extensively investigated in adipocytes, it is currently unknown whether UCP1 in the microenvironment of WAT such as adipose-tissue macrophages (ATM) could play a role in thermogenesis. Given the critical role of hypoxia in regulating obesity and insulin resistance, we challenged our myeloid-specific hypoxia-inducible factor-1α (Hif-1α) knockout (KO) mice to high fat diet and observed that these mice were not only protected from diet-induced obesity but also resistant to the body temperature changes upon cold exposure, the latter effect being completely lost upon surgical removal of WAT. Mechanistically, ATM deficient for HIF-1α generated heat production through increased expression of UCP1 and increased mitochondrial functions, all of which lead to an enhanced lipolysis in neighboring adipocytes. Lastly, we observed a number of co-localized areas between UCP-1 and CD68 in WAT of lean mice as well as individuals whereas such areas were not observed in an obese mice or human subjects. In conclusion, we demonstrate a novel mechanism by which ATM regulate obesity through UCP1-mediated heat generation. Overall design: The investigation of gene expression change in FACS-sorted adipose tissue macrophages (ATM) from hMRP8cre+ Hif-1αfl/fl ApoE-/- mice or hMRP8cre- Hif-1αfl/fl ApoE-/- mice."],"tag":["xref:PubMed:39473019"],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"Heat production from adipose-tissue macrophages regulates lipolysis and diet-induced obesity","description":"Heat production from adipose-tissue macrophages regulates lipolysis and diet-induced obesity","dates":{"last_updated":"2025-09-24","first_public":"2024-12-19"},"accession":"PRJNA599946","cross_references":{"GEO":["GSE143298"],"taxon":["10090"],"PubMed":["39473019"]}}