ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/091/SRR10868891/SRR10868891_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/079/SRR10868879/SRR10868879_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/087/SRR10868887/SRR10868887_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/083/SRR10868883/SRR10868883_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/080/SRR10868880/SRR10868880_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/089/SRR10868889/SRR10868889_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/085/SRR10868885/SRR10868885_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/088/SRR10868888/SRR10868888_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/076/SRR10868876/SRR10868876_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/084/SRR10868884/SRR10868884_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/090/SRR10868890/SRR10868890_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/076/SRR10868876/SRR10868876_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/088/SRR10868888/SRR10868888_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/083/SRR10868883/SRR10868883_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/082/SRR10868882/SRR10868882_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/086/SRR10868886/SRR10868886_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/077/SRR10868877/SRR10868877_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/085/SRR10868885/SRR10868885_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/077/SRR10868877/SRR10868877_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/089/SRR10868889/SRR10868889_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/079/SRR10868879/SRR10868879_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/091/SRR10868891/SRR10868891_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/081/SRR10868881/SRR10868881_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/082/SRR10868882/SRR10868882_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/078/SRR10868878/SRR10868878_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/086/SRR10868886/SRR10868886_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/080/SRR10868880/SRR10868880_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/090/SRR10868890/SRR10868890_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/078/SRR10868878/SRR10868878_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/081/SRR10868881/SRR10868881_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/087/SRR10868887/SRR10868887_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR108/084/SRR10868884/SRR10868884_2.fastq.gzprimary200OKGenomicsCBDM, Microbiology and Immunobiology, Harvard Medical Schoolhttps://www.ebi.ac.uk/ena/browser/view/PRJNA600478Mus musculusA distinct population of Foxp3+CD4+ regulatory T (Treg) cells promotes repair of acutely or chronically injured skeletal muscle. The accumulation of these cells depends critically on interleukin (IL)-33 produced by local mesenchymal stromal cells (mSCs). An intriguing physical association between muscle nerves, IL-33+ mSCs and Tregs has been reported, and begs a deeper exploration of this cell triumvirate. Here we evidence a striking proximity between IL-33+ muscle mSCs and both large-fiber nerve bundles and small-fiber sensory neurons report that muscle mSCs transcribe an array of genes encoding neuropeptides, neuropeptide receptors and other nerve-related proteins define muscle mSC subtypes that express both IL-33 and the receptor for the calcitonin-gene-related peptide (CGRP) and demonstrate that up- or down- tuning of CGRP signals augments or diminishes, respectively, IL-33 production by muscle mSCs and later accumulation of muscle Tregs. Indeed, a single injection of CGRP induced much of the genetic program elicited in mSCs early after acute skeletal muscle injury. These findings highlight neural/stromal/immune-cell cross-talk in tissue repair, suggesting future therapeutic approaches. Overall design: Gene expression profiles of muscle mesenchymal stromal cells from mice injected IP with CGRP or PBS (isolated at 4 or 8 hours post-injection). 4 biological replicates were obtained per experimental group.xref:PubMed:32102913ENA3.4.22.-, Multipotent Bone Marrow Stromal Cells, Mesenchymal Stem Cell, Laboratory, Calcium-binding protein in amniotic fluid 1, Mesenchymal Stem Cells, Mus domesticus, Stem Cells, Progenitor Cell, Stromal Cells, Mesenchymal Stromal Cells, Adipose Tissue Derived Mesenchymal Stromal Cell, Wharton's Jelly Cells, mini-ICE, CASP-14, Multipotent Mesenchymal Stromal Cell, House Mouse, Calgranulin-C, CGRP1, bone marrow stromal cells, stem cells, Roles, House, Stromal Cell, Concepts, Mus musculus domesticus, Wharton Jelly Cells, EN-RAGE, mesenchymal precursor cell, Mice, Wharton's Jelly Cell, mesenchymal stromal cell, Multipotent Mesenchymal Stromal Cells, CAAF1, Adipose Tissue-Derived Mesenchymal Stromal Cells, Adipose Derived Mesenchymal Stem Cells, 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Mesenchymal Stem Cell, Migration inhibitory factor-related protein 6, Mouse, Bone Marrow Stromal Cells, Muscle Tissue, Laboratory Mouse, CtnfalseRole of CGRP on muscle mesenchymal stromal cells in miceRole of CGRP on muscle mesenchymal stromal cells in mice2025-09-242020-02-12PRJNA600478GSE1434241009032102913