ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/082/SRR10953982/SRR10953982.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/084/SRR10953984/SRR10953984.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/083/SRR10953983/SRR10953983.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/081/SRR10953981/SRR10953981.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/085/SRR10953985/SRR10953985.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/086/SRR10953986/SRR10953986.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/088/SRR10953988/SRR10953988.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/080/SRR10953980/SRR10953980.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/087/SRR10953987/SRR10953987.fastq.gzprimary200OKGenomicsUniversity of Pennsylvaniahttps://www.ebi.ac.uk/ena/browser/view/PRJNA602736Mus musculusThe role of autophagy and metabolism in regulating the pluripotency of stem cells is not well understood. LAMP2A is a key gene in CMA. Here we compared transcriptomic changes in LAMP2A-overexpressed D3 versus control cells, as well as changes in LAMP2A-overexpressed cells with versus without DM-αKG treatment (4mM, 72 hrs). We found that overexpression of LAMP2A leads to decreased expression of genes related to stem cell differentiation, and this can be largely restored by DM-αKG supplement. This study provides a promising link for autophagy, metabolism, and pluripotency of stem cells. Overall design: RNA-Seq was performed to measure transcriptomes of D3 ES cells under three conditions: 1) cells stably expressed pCDH-control vector (VT), 2) LAMP2A overexpressed cells without (LAMP2A), and 3) with DM-αKG treatment (4 mM, 72 hrs) (LAMP2A+ DM-αKG).xref:PubMed:32703873ENAfalseRNA-seq analysis of transcriptomes in control and LAMP2A-overexpressed D3 ES cellsRNA-seq analysis of transcriptomes in control and LAMP2A-overexpressed D3 ES cells2025-09-242020-11-10PRJNA602736GSE1440931009032703873