ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/010/SRR10988310/SRR10988310.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/007/SRR10988307/SRR10988307.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/076/SRR10984176/SRR10984176.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/015/SRR10988315/SRR10988315.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/013/SRR10988313/SRR10988313.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/077/SRR10984177/SRR10984177.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/073/SRR10984173/SRR10984173.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/009/SRR10988309/SRR10988309.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/016/SRR10988316/SRR10988316.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/011/SRR10988311/SRR10988311.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/075/SRR10984175/SRR10984175.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/008/SRR10988308/SRR10988308.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/078/SRR10984178/SRR10984178.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/014/SRR10988314/SRR10988314.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/012/SRR10988312/SRR10988312.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/074/SRR10984174/SRR10984174.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/017/SRR10988317/SRR10988317.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/006/SRR10988306/SRR10988306.fastq.gzprimaryOK200GenomicsMURPf52University of Regensburghttps://www.ebi.ac.uk/ena/browser/view/PRJNA603674Pyrococcus furiosusxref:EuropePMC:PMC7830388In several archaea, the Cu-transporting ATPase and the copper chaperone are arranged in a conserved copper resistance gene cluster (cop), which also contains an additional gene, encoding for a DNA-binding transcriptional regulator. In previous studies, pf0739 has been proposed to be the copper-dependent regulator CopR in P. furiosus.Here, we have characterised the metal-sensing transcriptional regulator CopR by describing the influence of different metal ions on the DNA-binding of wild-type and domain-depleted pf0739 mutants. To learn about the CopR-regulated gene network in P. furiosus, we performed differential gene expression analysis (DGE) and chromatin immunoprecipitation in combination with high-throughput sequencing (ChIP-seq) under normal and 20 microM copper-shock conditions. By integrating the transcriptome and genome-wide binding data, we found that CopR binds to the upstream regions of many copper-induced genes. Activation of the exporting ATPase (PF0740) was confirmed using in vitro transcription assays and DNase I footprinting. Negative-stain transmission electron microscopy and image analysis by 2D class averaging revealed an octameric assembly formed from a tetramer of dimers similar to the published crystal structures from LrpA from P. furiosus and FFRP, an orthologue of Pyrococcus sp. OT3.ENAstrain, Sprain, Strain., cultivar, Strains, Sprains, Strains and Sprains, ecotyperegulator, copper homeostasis.falsePyrococcus furiosus strain:MURPf52CopR is a global regulator of copper homeostasis in Pyrococcus furiosus2025-09-242020-05-18PRJNA6036742261