{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/069/SRR10987369/SRR10987369.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/065/SRR10987365/SRR10987365.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/072/SRR10987372/SRR10987372.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/068/SRR10987368/SRR10987368.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/066/SRR10987366/SRR10987366.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/071/SRR10987371/SRR10987371.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/070/SRR10987370/SRR10987370.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/067/SRR10987367/SRR10987367.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/073/SRR10987373/SRR10987373.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR109/074/SRR10987374/SRR10987374.fastq.gz"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["National Cancer Institute"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA603835"],"tag":["xref:EuropePMC:PMC9112975","xref:EuropePMC:PMC7474616","xref:EuropePMC:PMC9226507"],"long_description":["Bacterial transcription is punctuated by RNA polymerase (RNAP) pausing that can provide time for regulatory events to occur. However, the genome-wide role of transcription elongation factors on pausing had not been examined in vivo. We determined the genome-wide effect of Bacillus subtilis NusG on RNAP pausing using RNET-seq, a nascent RNA sequencing protocol. NusG shifts RNAP to the post-translocation register, and induces pausing at ~1600 sites containing a conserved TTNTTT motif in the non-template DNA strand within the paused transcription bubble. NusG-dependent pausing was confirmed for all pause sites tested in vitro. NusG-dependent pausing in the ribD riboswitch provides time for cotranscriptional FMN binding, which decreases the concentration of FMN required for termination."],"repository":["ENA"],"description_synonyms":["Bacillus subtilis var. natto, Bacillus natto, Vibrio subtilis, Bacillus mesentericus, bacterial transcription, Bacillus subtilis subsp. natto, Bacillus subtilis8, Bacillus uniflagellatus., Natto Bacteria, Bacillus globigii, transcription from bacterial-type RNA polymerase promoter, Bacillus subtilis/Bacillus globigii, Bacillus subtilis (natto)"],"additional_accession":[]},"is_claimable":false,"name":"","description":"NusG-dependent transcription pausing in Bacillus subtilis","dates":{"last_updated":"2023-05-19","first_public":"2020-08-06"},"accession":"PRJNA603835","cross_references":{}}