ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR110/021/SRR11033421/SRR11033421.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR110/023/SRR11033423/SRR11033423.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR110/022/SRR11033422/SRR11033422.fastq.gzprimaryOK200GenomicsUniversity of Edinburghhttps://www.ebi.ac.uk/ena/browser/view/PRJNA605108Mus musculusxref:PubMed:35858557Hematopoietic stem cell (HSC) generation in the aorta-gonads-mesonephros region requires HSC specification signals from the surrounding microenvironment. In zebrafish, PDGF-B/PDGFRβ signaling controls hematopoietic stem/progenitor cell (HSPC) generation and is required in the HSC specification niche. Little is known about murine HSPC specification in vivo and whether PDGF-B/PDGFRβ is involved. Here we show that PDGFRβ is expressed in distinct perivascular stromal cell layers surrounding the mid-gestation dorsal aorta, and its deletion impairs hematopoiesis. We demonstrate that PDGFRβ+ cells play a dual role in murine hematopoiesis. They act in the aortic niche to support HSPCs, and in addition, PDGFRβ+ embryonic precursors give rise to a subset of HSPCs that persist into adulthood. These findings provide crucial information for the controlled production of these clinically important cells in vitro. Overall design: A C57/BL6 litter was analyzed (embryos pulled together in one sample)ENAfalsePDGFRβ+ cells play a dual role as hematopoietic precursors and niche cells during mouse ontogenyPDGFRβ+ cells play a dual role as hematopoietic precursors and niche cells during mouse ontogeny2025-09-242023-02-07PRJNA605108GSE1448371009035858557