ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR110/028/SRR11068828/SRR11068828.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR110/024/SRR11068824/SRR11068824.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR110/027/SRR11068827/SRR11068827.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR110/026/SRR11068826/SRR11068826.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR110/025/SRR11068825/SRR11068825.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR110/023/SRR11068823/SRR11068823.fastq.gzprimaryOK200GenomicsBioinformatics, Babraham Institutehttps://www.ebi.ac.uk/ena/browser/view/PRJNA606076Caenorhabditis elegansTo survive elevated temperatures, ectotherms adjust the fluidity of membranes by fine-tuning lipid desaturation levels in a process that has been previously described to be cell-autonomous. We have discovered that, in Caenorhabditis elegans, the neuronal over-expression of the master regulator of the conserved heat shock response (HSR), Hsf-1, causes extensive fat remodelling in peripheral tissues. These changes include a decrease in fat desaturase expression in the gut, and a shift in the saturation levels of fatty acids in the plasma membrane, in line with ectothermic adaptive responses. We have identified the cGMP receptor TAX-2/TAX-4 and TGF-β/BMP signalling, as key players in signalling across tissues. We also find that gradual increments in ambient temperature result in HSR activation exclusively in wild-type head neurons. This is the first study to suggest that a thermostat-based mechanism can non-cell autonomously coordinate membrane fluidity in response to warm temperatures across tissues in multicellular animals. Overall design: RNA-seq comparison between young adult N2 (WT) and AGD1289 (neuronal over-expression of HSF-1)xref:PubMed:34723964ENASCARB3, biological signaling, BODYFAT, HSFa, CT11259, ho, pun, Activity, bis(monoacylglycerol) hydrogen phosphate, M(2)LS1, GPIIIB, gcn, AtrII, Act-r, l(2)ft, fat, DPP-C, BDPLT10, hFat1, Dm Fat, TGFbeta-60A, TGFbeta, Hin-d, DmelCG5562, DmelCG9885, HGF, CDHF7, dHSFa, IL-6, DmelCG7904, PAS-4, Scarb3, CG3352, Cph1, GP3B, fatty tissue, l(2)60A-J, fat tissue, Dm-DPP, D-HSF, Dpp, DPP, adipose system, M(2)23AB, dlhC, Gbb-60A, Tissue, 79/18, CG1945, Fatty acid translocase, tgfb-60A, CG5748, blk, DmHSFa, BMP, Dm-Hsf, l(2)03091, GP4, signaling process, Glycoprotein IIIb, bodyfat, atrII, CPH, Atr, single organism signaling, signalling., xhsf1, DmelCG3352, l(3)j5A5, l(2)gd-1, Tgf-r, BcDNA:LD22582, TGF-b, R74677, Punt, TGF-beta, BcDNA.LD22582, Tg, l(2)gd2, CG5562, CG9885, dHSF, HSF, Hsf, CD36, ME5, CG7904, Tgfb-60, BSF2, DmHSF, Body Fat, vgr/60A, Platelet glycoprotein IV, Atr88CD, PASIV, Atr-II, l(2)22Fa, PUNT, PAS IV, l(3)10460, gbb-60A, hsf, Cph-1, Dm-GBB, CDHR8, l(2)k07918, l(2)k17036, Platelet collagen receptor, shv, LBPA, Gbb, GBB, Leukocyte differentiation antigen CD36, 60A, l(2)10638, CHDS7, l(2)gd-l, HSF1, Tgfbeta-60A, IFNB2, l(2)fd, signalling process, DmelCG5748, fatty depot, TGF-B, adipose, Faf, DmelCG1945, Ft, Put, Thrombospondin receptor, l(2)fat, l(2)24Da, GPIV, FAT, Fat, STK-C, SixtyA, General activity, l(2)79/18SCARB3, biological signaling, BODYFAT, HSFa, CT11259, ho, pun, Activity, bis(monoacylglycerol) hydrogen phosphate, M(2)LS1, GPIIIB, gcn, AtrII, Act-r, l(2)ft, fat, DPP-C, BDPLT10, hFat1, Dm Fat, TGFbeta-60A, TGFbeta, Hin-d, DmelCG5562, DmelCG9885, HGF, CDHF7, dHSFa, IL-6, DmelCG7904, PAS-4, Scarb3, CG3352, Cph1, GP3B, fatty tissue, l(2)60A-J, fat tissue, Dm-DPP, D-HSF, Dpp, DPP, adipose system, M(2)23AB, dlhC, Gbb-60A, Tissue, 79/18, CG1945, Fatty acid translocase, tgfb-60A, CG5748, blk, DmHSFa, BMP, Dm-Hsf, l(2)03091, GP4, signaling process, Glycoprotein IIIb, bodyfat, atrII, CPH, Atr, single organism signaling, signalling., xhsf1, DmelCG3352, l(3)j5A5, l(2)gd-1, Tgf-r, BcDNA:LD22582, TGF-b, R74677, Punt, TGF-beta, BcDNA.LD22582, Tg, l(2)gd2, CG5562, CG9885, dHSF, HSF, Hsf, CD36, ME5, CG7904, Tgfb-60, BSF2, DmHSF, Body Fat, vgr/60A, Platelet glycoprotein IV, Atr88CD, PASIV, Atr-II, l(2)22Fa, PUNT, PAS IV, l(3)10460, gbb-60A, hsf, Cph-1, Dm-GBB, CDHR8, l(2)k07918, l(2)k17036, Platelet collagen receptor, shv, LBPA, Gbb, GBB, Leukocyte differentiation antigen CD36, 60A, l(2)10638, CHDS7, l(2)gd-l, HSF1, Tgfbeta-60A, IFNB2, l(2)fd, signalling process, DmelCG5748, fatty depot, TGF-B, adipose, Faf, DmelCG1945, Ft, Put, Thrombospondin receptor, l(2)fat, l(2)24Da, GPIV, FAT, Fat, STK-C, SixtyA, General activity, l(2)79/18falseNeuronal HSF-1 activity coordinates fat desaturation across tissues in C. elegans via TGF-b/BMP signallingNeuronal HSF-1 activity coordinates fat desaturation across tissues in C. elegans via TGF-b/BMP signalling2025-09-242021-10-21PRJNA606076GSE145123623934723964