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Lab, Neuroscience of Aging and Depression, Center for Addiction and Mental Health"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA607505"],"scientific_name":["Mus musculus"],"tag":["xref:PubMed:34861977"],"long_description":["Major depressive disorder (MDD) is a severe psychiatric disorder, characterized by deficits in GABAergic and glutamatergic signaling in cortical brain regions. Unpredictable chronic mild stress (UCMS), a murine stress model, induces behavioural and neurobiological changes reminiscent of MDD. Despite increasing knowledge of the cellular complexity of the cortical microcircuitry, our understanding of how distinct cell-types are impacted by UCMS is extremely limited. We thus endeavoured to determine the effect of UCMS on Pyramidal (PYR) cells and three classes of interneurons: Somatostatin (SST), Parvalbumin (PV), and Vasoactive intestinal peptide-expressing (VIP) cells in the medial prefrontal cortex. C57Bl/6 mice, exposed to UCMS or control housing for five weeks, were assessed with a battery of anxiety- and depressive-like behavioural tests. Laser-capture microdissection was used to isolate samples of microcircuit cell-types, which underwent RNA-sequencing. UCMS-exposed mice showed significantly elevated emotionality, and isolate cell-types showed robust specificity. Cell-types exhibited unique transcriptomic profiles after UCMS. Briefly, PYR-cells showed decreased signaling and post-synaptic receptor expression, SST-cells showed increased activation of the unfolded protein response, PV-cells showed increased axonal microtubule expression, and VIP-cells showed increased apoptotic signaling and altered cytoskeletal gene expression. Co-expression analyses revealed that these changes were largely correlated across cell-types and that UCMS increased transcriptome-wide co-expression between PYR and both PV and SST cells. Overall, these findings suggest decreased PYR, SST, and VIP-cell function, elevated PV-cell function, and identify the dysregulation of PYR-cells as a central locus of UCMS-induced transcriptomic change. Further studies interrogating potential antidepressant-like activity of compounds reversing these cell-specific changes are warranted. Overall design: Examination of gene expression in mice exposed to chronic stress, versus controls, in four neuronal cell-types isolated from the medial prefrontal cortex."],"repository":["ENA"],"description_synonyms":["Automatic Data Processing., MGC130048, suprarenal cortex, dTAF[[II]]230, TAF[[II]]250, d230, SGCG_HUMAN, 35 kDa dystrophin-associated glycoprotein, SG-gamma, TAF200, A4, l(3)84Ab, dTAFII250, Computer Data Processing, BG:DS00004.13, Computer, TAFII-250, TAF250/230, EfW1, Cell, TYPE, SGCG, LGMD2C, dTAF230, DAGA4, dmTAF[[II]]230, TAFII250, Information Processing, Automatic Information, dmTAF1, Taf230, p230, Cortical Portion of the Kidney, 35DAG, Bar Code, anxiety, TAF[[II]]250/230, TFIID, sarcoglycan, CORTEX, Cortex of adrenal gland, MAM, gamma-SG, SCG3, KIDNEY, cortex of suprarenal gland, TAF250, Automatic, Taf[[II]]250, gamma sarcoglycan, Taf200, dTAF[[II]]250, TAF[[II]]230, TFIID TAF250, DMDA1, Optical Readers, Automatic Information Processing, cel, cell, Information, Cortical, Processing, Electronic Data, Taf1p, TAF[II]250, cortex glandulae suprarenalis, CG17603, gamma (35kDa dystrophin-associated glycoprotein), TAF[[II]], Data Processing, dTAF250, Codes, Bar Codes, DmelCG17603, DMDA, Automatic Data, Taf250, Electronic, SR3-5, 35kD dystrophin-associated glycoprotein, Computer Data, SCARMD2, gamma-sarcoglycan, Bar, Cortical Portion of Kidney, TAF, TAF230, Optical Reader, TAF1"],"name_synonyms":["Automatic Data Processing., MGC130048, suprarenal cortex, dTAF[[II]]230, TAF[[II]]250, d230, SGCG_HUMAN, 35 kDa dystrophin-associated glycoprotein, SG-gamma, TAF200, A4, l(3)84Ab, dTAFII250, Computer Data Processing, BG:DS00004.13, Computer, TAFII-250, TAF250/230, EfW1, Cell, TYPE, SGCG, LGMD2C, dTAF230, DAGA4, dmTAF[[II]]230, TAFII250, Information Processing, Automatic Information, dmTAF1, Taf230, p230, Cortical Portion of the Kidney, 35DAG, Bar Code, anxiety, TAF[[II]]250/230, TFIID, sarcoglycan, CORTEX, Cortex of adrenal gland, MAM, gamma-SG, SCG3, KIDNEY, cortex of suprarenal gland, TAF250, Automatic, Taf[[II]]250, gamma sarcoglycan, Taf200, dTAF[[II]]250, TAF[[II]]230, TFIID TAF250, DMDA1, Optical Readers, Automatic Information Processing, cel, cell, Information, Cortical, Processing, Electronic Data, Taf1p, TAF[II]250, cortex glandulae suprarenalis, CG17603, gamma (35kDa dystrophin-associated glycoprotein), TAF[[II]], Data Processing, dTAF250, Codes, Bar Codes, DmelCG17603, DMDA, Automatic Data, Taf250, Electronic, SR3-5, 35kD dystrophin-associated glycoprotein, Computer Data, SCARMD2, gamma-sarcoglycan, Bar, Cortical Portion of Kidney, TAF, TAF230, Optical Reader, TAF1"],"additional_accession":[]},"is_claimable":false,"name":"Chronic Stress Induces Coordinated Cortical Microcircuit Cell-Type Transcriptomic Changes Consistent With Altered Information Processing","description":"Chronic Stress Induces Coordinated Cortical Microcircuit Cell-Type Transcriptomic Changes Consistent With Altered Information Processing","dates":{"last_updated":"2025-09-24","first_public":"2022-02-19"},"accession":"PRJNA607505","cross_references":{"GEO":["GSE145521"],"taxon":["10090"],"PubMed":["34861977"]}}