ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR111/047/SRR11140647/SRR11140647_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR123/095/SRR12379495/SRR12379495_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR111/045/SRR11140645/SRR11140645_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR111/049/SRR11140649/SRR11140649_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR123/093/SRR12379493/SRR12379493_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR123/095/SRR12379495/SRR12379495_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR111/048/SRR11140648/SRR11140648_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR111/046/SRR11140646/SRR11140646_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR111/045/SRR11140645/SRR11140645_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR123/093/SRR12379493/SRR12379493_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR111/049/SRR11140649/SRR11140649_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR111/046/SRR11140646/SRR11140646_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR111/048/SRR11140648/SRR11140648_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR123/094/SRR12379494/SRR12379494_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR111/044/SRR11140644/SRR11140644_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR111/047/SRR11140647/SRR11140647_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR111/044/SRR11140644/SRR11140644_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR123/094/SRR12379494/SRR12379494_1.fastq.gzprimary200OKGenomicsBiomedical Genetics, University of Rochester Medical Centerhttps://www.ebi.ac.uk/ena/browser/view/PRJNA608062Mus musculusGenome-wide chromatin state underlies gene expression potential and cellular function. Epigenetic features and nucleosome positioning contribute to the accessibility of DNA, but widespread regulators of chromatin state are largely unknown. Our study investigates how coordination of ANP32E and H2A.Z contributes to genome-wide chromatin state in mouse fibroblasts. We define H2A.Z as a universal chromatin accessibility factor, and demonstrate that ANP32E antagonizes H2A.Z accumulation to restrict chromatin accessibility genome-wide. In the absence of ANP32E, H2A.Z accumulates at promoters in a hierarchical manner. H2A.Z initially localizes downstream of the transcription start site, and if H2A.Z is already present downstream, additional H2A.Z accumulates upstream. This hierarchical H2A.Z accumulation coincides with improved nucleosome positioning, heightened transcription factor binding, and increased expression of neighboring genes. Thus, ANP32E dramatically influences genome-wide chromatin accessibility through subtle refinement of H2A.Z patterns, providing a means to reprogram chromatin state and to hone gene expression levels. Overall design: Gene expression profiles, chromatin accessibility and CUT&Tag profiles in wildtype (WT) and Anp32e null mouse embryonic fibroblasts were generated by next-generation sequencing, in triplicates.xref:PubMed:33033242ENAnuclear chromatin, 2810018A15Rik, HITS-CLIP, High Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, Chromatin Immunoprecipitation Sequencing Chip, cytoplasmic chromatin, CPD1, ChIP-Chip, Chromatin Immuno precipitation Sequencing, wide/broad, Chromatin Immuno-precipitation, ATAC-seq assay, Genomes, ChIP, Chromatin Immunoprecipitation Paired End Tag, ATAC-seq, Cross Linking and Immunoprecipitation Followed by Deep Sequencing, Chromatin Immuno Precipitation Paired End Tag, Cross-Linking and Immunoprecipitation Followed by Deep Sequencing, mLANP-L, ChIP Sequencing, Chromatin Immunoprecipitation, CLIP-Seq, Chromatin Immuno-precipitation Sequencing, whole genome, broad, Assay for Transposase-Accessible Chromatin Using Sequencing, ChIP Exonuclease, ChIP-PET, ChIP-Seq, Sequencing, LANP-L, ChIP-Exo, Assay for Transposase Accessible Chromatin Using Sequencing, Chromatin Immunoprecipitation Paired-End Tag, wide, ChIA-PET., Chromatin Immuno-Precipitation Paired-End Tag, chromosome scaffold, ATAC-Seq, Chromatin Immunoprecipitation Sequencing-Chips, Chromatin Immunoprecipitation Sequencing-Chip, High-Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, LANPL, ChIP-Exonuclease, Chromatins, AI047746, AI326868nuclear chromatin, 2810018A15Rik, HITS-CLIP, High Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, Chromatin Immunoprecipitation Sequencing Chip, cytoplasmic chromatin, CPD1, ChIP-Chip, Chromatin Immuno precipitation Sequencing, wide/broad, Chromatin Immuno-precipitation, ATAC-seq assay, Genomes, ChIP, Chromatin Immunoprecipitation Paired End Tag, ATAC-seq, Cross Linking and Immunoprecipitation Followed by Deep Sequencing, Chromatin Immuno Precipitation Paired End Tag, Cross-Linking and Immunoprecipitation Followed by Deep Sequencing, mLANP-L, ChIP Sequencing, Chromatin Immunoprecipitation, CLIP-Seq, Chromatin Immuno-precipitation Sequencing, whole genome, broad, Assay for Transposase-Accessible Chromatin Using Sequencing, ChIP Exonuclease, ChIP-PET, ChIP-Seq, Sequencing, LANP-L, ChIP-Exo, Assay for Transposase Accessible Chromatin Using Sequencing, Chromatin Immunoprecipitation Paired-End Tag, wide, ChIA-PET., Chromatin Immuno-Precipitation Paired-End Tag, chromosome scaffold, ATAC-Seq, Chromatin Immunoprecipitation Sequencing-Chips, Chromatin Immunoprecipitation Sequencing-Chip, High-Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, LANPL, ChIP-Exonuclease, Chromatins, AI047746, AI326868falseGenome-wide Chromatin Accessibility is Restricted by ANP32E [ATAC-seq]Genome-wide Chromatin Accessibility is Restricted by ANP32E [ATAC-seq]2025-09-242020-09-11PRJNA608062GSE1457031009033033242