ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR115/026/SRR11550226/SRR11550226.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR115/022/SRR11550222/SRR11550222.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR115/027/SRR11550227/SRR11550227.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR115/023/SRR11550223/SRR11550223.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR115/030/SRR11550230/SRR11550230.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR115/025/SRR11550225/SRR11550225.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR115/028/SRR11550228/SRR11550228.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR115/020/SRR11550220/SRR11550220.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR115/024/SRR11550224/SRR11550224.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR115/031/SRR11550231/SRR11550231.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR115/029/SRR11550229/SRR11550229.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR115/021/SRR11550221/SRR11550221.fastq.gzprimaryOK200GenomicsBioinformatics Core, MDI Biological Laboratoryhttps://www.ebi.ac.uk/ena/browser/view/PRJNA625528Caenorhabditis elegansxref:PubMed:36273621xref:EuropePMC:PMC9677334This experiment compares gene expression and polysome profiling between a strain where glh-1 is present - DUP64 glh-1(sam24[glh-1::gfp::3xFlag]) I and a strain where the glh-1 coding sequence has been deleted DUP144 glh-1(sam65[Δglh-1::gfp::3xFlag]) I Overall design: For each of three replicates, 25 plates of each strain were used to inoculate 1L of S Media containing 5 grams of freeze-dried OP50 (LabTie, Leiden, The Netherlands). Liquid cultures were incubated in 4 1L beveled flasks at 20° C until worms were gravid, at which point embryos were harvested, washed, bleach-treated (Montserrat et al. 2012) and hatched overnight. The synchronized L1-staged worms were used to inoculate another 1L of S Media with OP50 and were grown for approximately 40 hours until the majority reached the young adult stage, where they were harvested, washed, pelleted and flash frozen in 1-ml aliquots.ENAfalseAnalysis of gene expression and polysome profiling in the presence and absence of glh-1 in C. ElegansAnalysis of gene expression and polysome profiling in the presence and absence of glh-1 in C. Elegans2025-09-242022-11-24PRJNA625528GSE148737623936273621