ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR116/014/SRR11682414/SRR11682414_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR116/022/SRR11682422/SRR11682422_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR116/010/SRR11682410/SRR11682410_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR116/018/SRR11682418/SRR11682418_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR116/016/SRR11682416/SRR11682416_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR116/023/SRR11682423/SRR11682423_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR116/020/SRR11682420/SRR11682420_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR116/011/SRR11682411/SRR11682411_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR116/021/SRR11682421/SRR11682421_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR116/015/SRR11682415/SRR11682415_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR116/019/SRR11682419/SRR11682419_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR116/013/SRR11682413/SRR11682413_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR116/010/SRR11682410/SRR11682410_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR116/019/SRR11682419/SRR11682419_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR116/025/SRR11682425/SRR11682425_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR116/022/SRR11682422/SRR11682422_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR116/012/SRR11682412/SRR11682412_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR116/020/SRR11682420/SRR11682420_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR116/016/SRR11682416/SRR11682416_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR116/024/SRR11682424/SRR11682424_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR116/013/SRR11682413/SRR11682413_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR116/017/SRR11682417/SRR11682417_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR116/014/SRR11682414/SRR11682414_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR116/025/SRR11682425/SRR11682425_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR116/017/SRR11682417/SRR11682417_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR116/023/SRR11682423/SRR11682423_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR116/015/SRR11682415/SRR11682415_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR116/024/SRR11682424/SRR11682424_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR116/021/SRR11682421/SRR11682421_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR116/018/SRR11682418/SRR11682418_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR116/012/SRR11682412/SRR11682412_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR116/011/SRR11682411/SRR11682411_1.fastq.gzprimaryOK200GenomicsPathology, UT Southwestern Medical Centerhttps://www.ebi.ac.uk/ena/browser/view/PRJNA630077Mus musculusBackground: NLRP12 is a cytosolic pattern recognition receptor in the family of NOD-like receptor. NLRP12 has been shown to suppress colorectal tumorigenesis. However, the precise mechansim of NLRP12-mediated regualtion of colorectal cancer is unknown. Results: RNAseq data demonstrate higher expression of oncogenes, matrix degrading enzymes such as matrix metaloproteinases (MMPs), and genes involved in tumor invasion in Nlrp12-/- compared to wild-type tumors. Notably, several of the genes highly induced in Nlrp12-/- tumors are regulated by the Wnt/b-catenin pathway. Conclusion: NLRP12 negatively regualtes the Wnt/b-catenin pathway to suppress colorectal cancer progression and invasion. Overall design: Methods: Colorectal tumors were induced in wild-type and Nlrp12-/- mice with a single injection (i.p.) of azoxymethane followed by 3 cycles of 2.5% dextran sodium sulphate administration for 5 days in each cycle. Tumors were collected 12 weeks post azoxymethane treatment. RNA was isolated from tumors and analyzed by RNAseq.ENAfalseRNAseq analysis of gene expression in colorectal tumors of wild-type and Nlrp12-/- miceRNAseq analysis of gene expression in colorectal tumors of wild-type and Nlrp12-/- mice2025-09-242020-10-02PRJNA630077GSE14976910090