{"database":"ENA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Bioinformatics and Biostatistics Core, Joslin Diabetes Center"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA631719"],"tag":["xref:PubMed:33674410"],"long_description":["To define the senescence-associated secretory phenotype (SASP) of beta-cells, we used conditioned media (CM) generated from bleomycin-treated MIN6 cells and from senescent (beta-Gal-positive) primary beta-cells. In order to culture senescent beta-cells, we isolated islet, FACS-sorted them into beta-Gal-positive and negative populations, excluding immune cells through negative selection of CD45-positive and CD11beta-positive cells. For both the MIN6 and primary beta-cell models, we cultured cells in serum-free media to generate CM for proteomic analysis using the aptamer-based SomaScan platform. Overall design: After FACS-sorting, beta-Gal-positive and negative primary beta-cells were plated separately in chamber slides or 96-well plates and incubated overnight at 37C and 7% CO2 in serum-free islet media to generate CM. Bleomycin-treated and control MIN6 cells were cultured for 24 hours in serum-free MIN6 media to generate CM. CM was analyzed using SomaScan at the Genomics Proteomics Core at Beth Israel Deaconess Medical Center."],"repository":["ENA"],"name_synonyms":["Txn1, l(3)rK137, SASP, e(-), DELTA AND GAMMA, Gene, protein, electron, betaTub1, 0763/13, CG17228, l(3)10419, 1135/09, 1135/07, dmTAF8, B1t, DmelCG17228, B-spec, betaspec, responsivity, SASPase, HL-VIII, 0451/09, Gene Products, anon-WO0140519.15, F15E12.6, Prosp, reactivity, PROS, F15E12_6, 0244/09, 0585/13, betatub(56D), kus, beta1tub, CG9277, MUB3_18, MUNO, Elektron, beta[[1]] tubulin, DROPROSA, CG7128, beta1t, T, beta-particle, proteins, MUB3.18, l(3)j6E2, anon-EST:fe1B3, l(3)rH013, 671/2, 1t, prod, 0320/10, e, Prodos, DMPROSPER, e-, beta1Tub, SPEC8, CG5870, Taps, TAF, 0563/18, 0671/02, l(3)j12C8, beta-tubulin56D, Spec-beta, ADF, Dmbeta1, BETA 56D, ATL-derived factor, l(3)rO534, PRO, Pro, Proteins, Tubulin, i168, Trx, TRX, CYS, Cell, l(3)rJ806, Pros, l(3)rL433, PROS-1, PROS-2, l(1)G0108, l(3)rI160, native protein, 0441/16, Surface-associated sulphydryl protein, pro, 1316/02, Protein, betaSpec, TFIID, BcDNA:HL08040, FLOWERING TIME CONTROL PROTEIN FCA ALPHA, TRDX, b-Spec, and GLY protein 2, MET, ATCMPG1, ATCMPG2, beta-tub, DmelCG5870, l(1)G0074, and GLY protein 1, l(1)G0198, Beta <eudicots>, negatron, Voila, Protein., beta[[1]]-tubulin, 0664/07, 1167/13, b spectrin, FCAALL.331, beta1, beta56D, DmelCG9277, TAF[[II]], beta, beta1-tub, beta(-), DTB2, Tub, l(3)rK204, Protein Gene Products, Gene Proteins, beta-Tub, DmelCG7128, beta-spec, i6, DL4180C, BETA, TRX1, l(1)16Fg, response, beta-Tub56D, TAF8, 0989/01, Spec, betaTub, Specbeta, beta1-Tubulin, pds"],"description_synonyms":["Txn1, l(3)rK137, SASP, e(-), DELTA AND GAMMA, Gene, protein, electron, betaTub1, 0763/13, CG17228, l(3)10419, 1135/09, 1135/07, dmTAF8, B1t, DmelCG17228, B-spec, betaspec, responsivity, SASPase, HL-VIII, 0451/09, Gene Products, anon-WO0140519.15, F15E12.6, Prosp, reactivity, PROS, F15E12_6, 0244/09, 0585/13, betatub(56D), kus, beta1tub, CG9277, MUB3_18, MUNO, Elektron, beta[[1]] tubulin, DROPROSA, CG7128, beta1t, T, beta-particle, proteins, MUB3.18, l(3)j6E2, anon-EST:fe1B3, l(3)rH013, 671/2, 1t, prod, 0320/10, e, Prodos, DMPROSPER, e-, beta1Tub, SPEC8, CG5870, Taps, TAF, 0563/18, 0671/02, l(3)j12C8, beta-tubulin56D, Spec-beta, ADF, Dmbeta1, BETA 56D, ATL-derived factor, l(3)rO534, PRO, Pro, Proteins, Tubulin, i168, Trx, TRX, CYS, Cell, l(3)rJ806, Pros, l(3)rL433, PROS-1, PROS-2, l(1)G0108, l(3)rI160, native protein, 0441/16, Surface-associated sulphydryl protein, pro, 1316/02, Protein, betaSpec, TFIID, BcDNA:HL08040, FLOWERING TIME CONTROL PROTEIN FCA ALPHA, TRDX, b-Spec, and GLY protein 2, MET, ATCMPG1, ATCMPG2, beta-tub, DmelCG5870, l(1)G0074, and GLY protein 1, l(1)G0198, Beta <eudicots>, negatron, Voila, Protein., beta[[1]]-tubulin, 0664/07, 1167/13, b spectrin, FCAALL.331, beta1, beta56D, DmelCG9277, TAF[[II]], beta, beta1-tub, beta(-), DTB2, Tub, l(3)rK204, Protein Gene Products, Gene Proteins, beta-Tub, DmelCG7128, beta-spec, i6, DL4180C, BETA, TRX1, l(1)16Fg, response, beta-Tub56D, TAF8, 0989/01, Spec, betaTub, Specbeta, beta1-Tubulin, pds"],"additional_accession":[]},"is_claimable":false,"name":"SASP signature of beta-cells includes pro-inflammatory and stress response proteins","description":"SASP signature of beta-cells includes pro-inflammatory and stress response proteins","dates":{"last_updated":"2023-05-19","first_public":"2021-04-08"},"accession":"PRJNA631719","cross_references":{"GEO":["GSE150285"],"PubMed":["33674410"]}}