ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/070/SRR12090570/SRR12090570.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/053/SRR12090553/SRR12090553.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/092/SRR12090592/SRR12090592.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/036/SRR12090536/SRR12090536.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/027/SRR12090627/SRR12090627.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/044/SRR12090644/SRR12090644.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/075/SRR12090575/SRR12090575.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/058/SRR12090558/SRR12090558.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/049/SRR12090649/SRR12090649.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/097/SRR12090597/SRR12090597.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/066/SRR12090666/SRR12090666.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/014/SRR12090614/SRR12090614.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/036/SRR12090636/SRR12090636.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/031/SRR12090631/SRR12090631.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/005/SRR12090605/SRR12090605.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/049/SRR12090549/SRR12090549.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/058/SRR12090658/SRR12090658.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/061/SRR12090661/SRR12090661.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/061/SRR12090561/SRR12090561.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/083/SRR12090583/SRR12090583.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/084/SRR12090584/SRR12090584.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/035/SRR12090635/SRR12090635.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/062/SRR12090562/SRR12090562.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/057/SRR12090657/SRR12090657.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/018/SRR12090618/SRR12090618.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/040/SRR12090640/SRR12090640.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/019/SRR12090619/SRR12090619.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/048/SRR12090648/SRR12090648.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/040/SRR12090540/SRR12090540.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/026/SRR12090626/SRR12090626.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/043/SRR12090643/SRR12090643.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/010/SRR12090610/SRR12090610.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/060/SRR12090660/SRR12090660.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/059/SRR12090559/SRR12090559.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/048/SRR12090548/SRR12090548.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/076/SRR12090576/SRR12090576.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/082/SRR12090582/SRR12090582.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/065/SRR12090565/SRR12090565.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/071/SRR12090571/SRR12090571.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/032/SRR12090532/SRR12090532.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/080/SRR12090580/SRR12090580.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/039/SRR12090639/SRR12090639.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/028/SRR12090628/SRR12090628.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/062/SRR12090662/SRR12090662.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/091/SRR12090591/SRR12090591.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/002/SRR12090602/SRR12090602.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/054/SRR12090654/SRR12090654.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/057/SRR12090557/SRR12090557.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/068/SRR12090568/SRR12090568.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/029/SRR12090629/SRR12090629.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/046/SRR12090646/SRR12090646.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/067/SRR12090567/SRR12090567.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/073/SRR12090573/SRR12090573.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/090/SRR12090590/SRR12090590.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/051/SRR12090651/SRR12090651.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/053/SRR12090653/SRR12090653.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/001/SRR12090601/SRR12090601.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/000/SRR12090600/SRR12090600.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/074/SRR12090574/SRR12090574.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/066/SRR12090566/SRR12090566.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/045/SRR12090645/SRR12090645.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/052/SRR12090652/SRR12090652.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/037/SRR12090637/SRR12090637.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/042/SRR12090542/SRR12090542.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/081/SRR12090581/SRR12090581.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/047/SRR12090547/SRR12090547.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/064/SRR12090564/SRR12090564.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/069/SRR12090569/SRR12090569.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/016/SRR12090616/SRR12090616.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/055/SRR12090655/SRR12090655.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/086/SRR12090586/SRR12090586.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/003/SRR12090603/SRR12090603.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/038/SRR12090638/SRR12090638.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/025/SRR12090625/SRR12090625.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/042/SRR12090642/SRR12090642.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/094/SRR12090594/SRR12090594.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/047/SRR12090647/SRR12090647.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/050/SRR12090650/SRR12090650.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/020/SRR12090620/SRR12090620.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/069/SRR12090669/SRR12090669.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/050/SRR12090550/SRR12090550.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/038/SRR12090538/SRR12090538.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/072/SRR12090572/SRR12090572.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/039/SRR12090539/SRR12090539.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/063/SRR12090663/SRR12090663.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/078/SRR12090578/SRR12090578.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/034/SRR12090634/SRR12090634.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/034/SRR12090534/SRR12090534.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/012/SRR12090612/SRR12090612.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/064/SRR12090664/SRR12090664.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/056/SRR12090556/SRR12090556.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/063/SRR12090563/SRR12090563.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/077/SRR12090577/SRR12090577.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/056/SRR12090656/SRR12090656.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/011/SRR12090611/SRR12090611.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/041/SRR12090541/SRR12090541.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/099/SRR12090599/SRR12090599.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/033/SRR12090633/SRR12090633.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/033/SRR12090533/SRR12090533.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/085/SRR12090585/SRR12090585.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/055/SRR12090555/SRR12090555.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/041/SRR12090641/SRR12090641.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/004/SRR12090604/SRR12090604.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/032/SRR12090632/SRR12090632.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/015/SRR12090615/SRR12090615.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/098/SRR12090598/SRR12090598.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/087/SRR12090587/SRR12090587.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/021/SRR12090621/SRR12090621.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/037/SRR12090537/SRR12090537.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/093/SRR12090593/SRR12090593.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/054/SRR12090554/SRR12090554.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/060/SRR12090560/SRR12090560.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/043/SRR12090543/SRR12090543.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/035/SRR12090535/SRR12090535.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/046/SRR12090546/SRR12090546.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/017/SRR12090617/SRR12090617.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/079/SRR12090579/SRR12090579.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/009/SRR12090609/SRR12090609.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/024/SRR12090624/SRR12090624.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/065/SRR12090665/SRR12090665.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/013/SRR12090613/SRR12090613.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/068/SRR12090668/SRR12090668.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/007/SRR12090607/SRR12090607.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/089/SRR12090589/SRR12090589.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/051/SRR12090551/SRR12090551.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/006/SRR12090606/SRR12090606.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/023/SRR12090623/SRR12090623.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/045/SRR12090545/SRR12090545.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/095/SRR12090595/SRR12090595.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/067/SRR12090667/SRR12090667.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/008/SRR12090608/SRR12090608.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/022/SRR12090622/SRR12090622.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/052/SRR12090552/SRR12090552.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/088/SRR12090588/SRR12090588.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/030/SRR12090630/SRR12090630.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/059/SRR12090659/SRR12090659.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/096/SRR12090596/SRR12090596.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR120/044/SRR12090544/SRR12090544.fastq.gzprimaryOK200GenomicsDuke Universityhttps://www.ebi.ac.uk/ena/browser/view/PRJNA641875Homo sapiensThe AP-1 transcription factor dimer contributes to many biological processes and environmental responses. The many ways that AP-1 subunits can dimerize has posed a challenge to understanding its regulatory function. To determine patterns of AP-1 dimerization genome-wide, we definitively establish the genome-wide binding patterns and the enhancer function of five AP-1 subunits. We find limited evidence for strong dimerization preferences between specific subunits. In particular, our analysis suggests that canonical AP-1 motifs have the potential to recruit all AP-1 subunits to genomic sites that also have hallmarks of strong enhancer activity. We term those sites AP-1 hotspots. AP-1 hotspots function as a ‘hub' for AP-1 subunit binding that elicits changes in cell-type specific AP-1-mediated gene expression. AP-1 hotspots are more predictive of genomic responses to glucocorticoid signaling than super enhancers, and are particularly enriched in disease-associated genetic variants. Together, these results support a model that promiscuous convergence of many subunits to common genomic locations potentiates AP-1-mediated transcription in a cell-type specific fashion. Overall design: ap1_flagtag: A549-derived cell lines with a FLAG epitope inserted at the C-terminus of each of seven AP-1 family members: c-FOS, FOSL1, FOSL2, FOSB, c-JUN, JUNB, and JUND. Directed precise insertion of each FLAG sequence via homologous recombination, using CRISPR/Cas9 to introduce a double strand break in the A549 genome and co-delivering a donor plasmid containing the FLAG tag and a puromycin resistance gene. After selecting for edited cells with puromycin, Cre recombinase was delivered to remove the resistance genexref:PubMed:33674350ENAfalseAP-1 subunits converge promiscuously at enhancers to potentiate transcriptionAP-1 subunits converge promiscuously at enhancers to potentiate transcription2025-09-242021-03-06PRJNA641875GSE153254960633674350