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Studies Division Genomics Laboratory, Health Canada, Government of Canadahttps://www.ebi.ac.uk/ena/browser/view/PRJNA642125Homo sapiensxref:PubMed:33476716In this study we used RNA-seq to characterize the dose-response for global transcriptomic alterations induced by BPA, BPF, BPS and TBBA in H9 (WA09) human embryonic stem (hES) cells. To assess the relative biological activity and potency of BPA and structural analogs BPS, BPF and TBBP, we examined the stem cell transcriptome in H9 hES cells following exposure to these chemicals at concentrations that covered greater than three orders of magnitude (1 microM to >100 microM). These exposure levels are considered to be near human relevant and range from noncytotoxic to overtly toxic. Overall, this study provides basic mechanistic information about the effects of BPA and structural analogs BPS, BPF and TBBP on the transcriptome of hES cells and demonstrates how these data can be applied to derive benchmark dose modeling (BMD) information useful for comparing relative potencies of these structurally related chemicals. Overall design: Examination of the stem cell transcriptomes in H9 hES cells following their exposure to BPA and structural analogs BPS, BPF and TBBP at several concentrations.ENAfalseTranscriptomics and relative potency assessments using Bench Mark Dose of Bisphenol A, Bisphenol S, Bisphenol F, and 3,3',5,5'-Tetrabromobisphenol A in H9 human embryonic stem cellsTranscriptomics and relative potency assessments using Bench Mark Dose of Bisphenol A, Bisphenol S, Bisphenol F, and 3,3',5,5'-Tetrabromobisphenol A in H9 human embryonic stem cells2025-09-242021-07-21PRJNA642125GSE153320960633476716