ENAGenomicsLiao Lab, Center for Regenerative Medicine, Massachusetts General Hospitalhttps://www.ebi.ac.uk/ena/browser/view/PRJNA644319Danio rerioxref:PubMed:33234718We report transcriptional profiles of 4hpf zebrafish embryos from either wild-type or a CRISPR-targeted irf6 loss-of-function mutant. Irf6 is a transcription factor implicated in syndromic and nonsyndromic forms of cleft palate. To define critical genes involved in palate development, we harvested RNA from irf6-/- embryos before an embryonic lethal stage in zebrafish and carried out RNA-sequencing and subsequent analysis. We identify top differentially expressed genes that include genes involved in periderm development, genes expressed in developmental processes (such as Fgfs and Wnt-pathway genes), and genes involved in orofacial clefting. We identify a crucial gene esrp1 that we chose for further validation and downstream analysis. Overall design: mRNA profiles of 4hpf wild-type or irf6-/- zebrafish embryos.ENAzebra fish, leopard danio, Transcriptome, AI876454, Zebra Fish, determination, Transcriptome Profile, Expression Profiles, Gene Expression Profile, Gene, Profiles, VWS1, PPS1, Gene Expression, zebra danio, Expression Signatures, Gene Expression Signatures, chemical analysis, Danio rerio frankei, Brachydanio rerio, VWS, Gene Expression Signature, Cyprinus rerio, Expression Profile, D. rerios, Transcriptome Profiles, Embryonic, Zebra, Danio rerio, Danio frankei, Zebrafishes, Profile, OFC6, LPS, PPS, Fishes, mirf6, danio, Brachydanio rerio frankei., Zebra danios, Signatures, xIRF-6, E230028I05Rik, Zebra danio, Expression Signature, Gene Expression Profiles, B. rerio, Zebra Fishes, Transcriptomes, D. rerio, assay, Signature, PIT, zebrafishzebra fish, leopard danio, Transcriptome, AI876454, Zebra Fish, determination, Transcriptome Profile, Expression Profiles, Gene Expression Profile, Gene, Profiles, VWS1, PPS1, Gene Expression, zebra danio, Expression Signatures, Gene Expression Signatures, chemical analysis, Danio rerio frankei, Brachydanio rerio, VWS, Gene Expression Signature, Cyprinus rerio, Expression Profile, D. rerios, Transcriptome Profiles, Embryonic, Zebra, Danio rerio, Danio frankei, Zebrafishes, Profile, OFC6, LPS, PPS, Fishes, mirf6, danio, Brachydanio rerio frankei., Zebra danios, Signatures, xIRF-6, E230028I05Rik, Zebra danio, Expression Signature, Gene Expression Profiles, B. rerio, Zebra Fishes, Transcriptomes, D. rerio, assay, Signature, PIT, zebrafishfalseEmbryonic transcriptome analysis of wt vs irf6-/- zebrafish at 4hpfEmbryonic transcriptome analysis of wt vs irf6-/- zebrafish at 4hpf2025-09-242020-12-12PRJNA644319GSE153828795533234718