ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR121/090/SRR12152390/SRR12152390_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR121/087/SRR12152387/SRR12152387_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR121/089/SRR12152389/SRR12152389_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR121/088/SRR12152388/SRR12152388_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR121/090/SRR12152390/SRR12152390_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR121/089/SRR12152389/SRR12152389_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR121/087/SRR12152387/SRR12152387_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR121/088/SRR12152388/SRR12152388_2.fastq.gzprimaryOK200GenomicsShanghai Institute of Materia Medica Chinese Academy Scienceshttps://www.ebi.ac.uk/ena/browser/view/PRJNA644345Pseudomonas aeruginosawe performed ChIP-seq assays to identify in vivo targets of GltR. The plasmid mini-gltR-flag-lacz was constructed and the resultant plasmid was fused to P. aeruginosa strain PAO1, yielding PAO1/mini-gltR-flag-lacz. We investigated GltR-binding to the chromosome of PAO1 during growth with glucose by ChIP-Seq. Sequence reads obtained from three independent ChIP-Seq experiments using anti-flag antibody and mapped to the P. aeruginosa PAO1 genome.Using the MACS software,we identified 55 enriched loci (q-value 3-fold, but were absent in control sample conducted without anti-flag antibody. Overall design: Perform a genome-wide screen for GltR binding sites.pathogen:prioritypathogen:bacteriumpathogenxref:PubMed:33670077bacteriaENAHITS-CLIP, High Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, Chromatin Immunoprecipitation Sequencing Chip, ChIP-Chip, Chromatin Immuno precipitation Sequencing, Chromatin Immuno-precipitation, determination, ChIP, Chromatin Immunoprecipitation Paired End Tag, Cross Linking and Immunoprecipitation Followed by Deep Sequencing, Chromatin Immuno Precipitation Paired End Tag, Cross-Linking and Immunoprecipitation Followed by Deep Sequencing, ChIP Sequencing, Chromatin Immunoprecipitation, CLIP-Seq, Chromatin Immuno-precipitation Sequencing, whole genome, Assay for Transposase-Accessible Chromatin Using Sequencing, ChIP Exonuclease, ChIP-PET, ChIP-Seq, Sequencing, ChIP-Exo, Assay for Transposase Accessible Chromatin Using Sequencing, Chromatin Immunoprecipitation Paired-End Tag, Chromatin Immuno-Precipitation Paired-End Tag, chemical analysis, ATAC-Seq, Chromatin Immunoprecipitation Sequencing-Chips, Chromatin Immunoprecipitation Sequencing-Chip, High-Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, assay, ChIP-Exonuclease, ChIA-PET, Genomes.HITS-CLIP, High Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, Chromatin Immunoprecipitation Sequencing Chip, ChIP-Chip, Chromatin Immuno precipitation Sequencing, Chromatin Immuno-precipitation, determination, ChIP, Chromatin Immunoprecipitation Paired End Tag, Cross Linking and Immunoprecipitation Followed by Deep Sequencing, Chromatin Immuno Precipitation Paired End Tag, Cross-Linking and Immunoprecipitation Followed by Deep Sequencing, ChIP Sequencing, Chromatin Immunoprecipitation, CLIP-Seq, Chromatin Immuno-precipitation Sequencing, whole genome, Assay for Transposase-Accessible Chromatin Using Sequencing, ChIP Exonuclease, ChIP-PET, ChIP-Seq, Sequencing, ChIP-Exo, Assay for Transposase Accessible Chromatin Using Sequencing, Chromatin Immunoprecipitation Paired-End Tag, Chromatin Immuno-Precipitation Paired-End Tag, chemical analysis, ATAC-Seq, Chromatin Immunoprecipitation Sequencing-Chips, Chromatin Immunoprecipitation Sequencing-Chip, High-Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, assay, ChIP-Exonuclease, ChIA-PET, Genomes.falseChIP-seq analysis reveals the direct targets of GltR in the P. aeruginosa genomeChIP-seq analysis reveals the direct targets of GltR in the P. aeruginosa genome2025-09-242021-02-25PRJNA644345GSE15384828733670077