ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR124/096/SRR12443296/SRR12443296_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR124/098/SRR12443298/SRR12443298_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR124/094/SRR12443294/SRR12443294_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR124/093/SRR12443293/SRR12443293_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR124/099/SRR12443299/SRR12443299_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR124/098/SRR12443298/SRR12443298_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR124/096/SRR12443296/SRR12443296_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR124/095/SRR12443295/SRR12443295_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR124/093/SRR12443293/SRR12443293_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR124/099/SRR12443299/SRR12443299_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR124/097/SRR12443297/SRR12443297_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR124/095/SRR12443295/SRR12443295_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR124/097/SRR12443297/SRR12443297_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR124/094/SRR12443294/SRR12443294_2.fastq.gzprimaryOK200GenomicsNational Research Institute for Family Planninghttps://www.ebi.ac.uk/ena/browser/view/PRJNA656893Currently, RESCUE RNA base editor proposes great potential for treating genetic diseases at transcription level. The relatively high off-target events are one of the main constrains for precisely RNA base editing mediated by RESCUE system. Here, we developed a new RNA base editor, ecRESCUE, fusing by inducible stabilization protein ecDHFR DD at the C-terminal of RESCUE system, respectively. The ecRESCUE RNA base editors are used for endogenous mRNA editing and off-target analysis. The results showed that the ecRESCUE RNA base editor markedly reduced its off-target SNP numbers without affecting RNA A-to-I and C-to-U base editing efficiency. Taken together, we developed an ecDHFR DD-regulated RESCUE system, ecRESCUE, with reduced RNA off-targeting activity.ENAfalseDevelop an ecDHFR-regulated RESCUE system, ecRESCUE, with reduced RNA off-targeting activity2023-05-172020-10-21PRJNA656893