{"database":"ENA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["PNNL"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA660617"],"scientific_name":["Homo sapiens"],"long_description":["The purpose is to obtain samples for mRNA analysis in primary normal human bronchial epithelial cells cultured with or without macrophage cells (+/- Mac) derived from primary peripheral blood monnumclear cells Overall design: Influenza A/Vietnam/1203/2004 (H5N1, VN1203) or influenza A/California/04/2009 (H1N1, CA04) viral stocks were used to infect NHBE cells at a multiplicity of infection (MOI) of 1. Infections were allowed to progress for 1 hour. Next, 500,000 peripheral blood mononuclear cell (PBMC)-derived macrophages were place onto each apical transwell surface in a final volume of 500 ul to create co­ cultures. The co-cultures at a ratio of approximately 2 NHBE cells for every PBMC-derived macrophage were allowed to progress for an additional 11, 15 and 23 hours respectively (12, 16, 24 hours post-infection). An n=5 was used for each condition, which included infection with either H1N1, H5N1, or mock uninfected, of either NHBE-only monocultures or NHBE + PBMC-macrophage co-cultures. In total this infection scheme represented a total of 90 samples for microarray analysis. At the specified time points, 1 ml of TRlzol RNA isolation reagent (Thermo Fischer, 15596-016) that lead to inactivation of samples."],"repository":["ENA"],"description_synonyms":["Transitional Epithelial Cell, Transitional, macrophagocyte, SPH116, Squamous Cells, BEST:GH10550, Leukocyte, human being, microphthalmos bilateral, Epithelial Cell, Peripheral Blood Mononuclear Cells, Monocyte Derived Macrophages, typical plasmatocyte, AconM, Modern, mAcon, CG6457, Adenomatous, Microphthalmia with colobomatous cyst, Glandular Epithelial, Monocyte Derived, Squamous, Glandular, colobomatous orbital cyst, monocyte-derived macrophage, PBMC Peripheral Blood Mononuclear Cells, Transitional Epithelial, Cell, i204, Human, microphthalmia associated with colobomatous cyst, membrane attack complex protein beta2 chain, Mononuclear Leukocyte., Monocyte-Derived, ACON-1, Squamous Epithelial Cells, DmelCG6457, Cuboidal Glandular Epithelial Cells, Microphthalmia - anophthalmia - coloboma, Homo sapiens, macrophage, AIR, MAC, Monocyte-Derived Macrophage, SP116, Peripheral Blood Mononuclear Cell, TCC, Columnar Glandular Epithelial Cells, DmelCG9244, agranular plasmatocyte, Man, Squamous Epithelial, Monocyte-Derived Macrophages, Epithelial, Macrophage, average, Adenomatous Epithelial Cell, Peripheral Blood Human Mononuclear Cells, Adenomatous Epithelial Cells, Squamous Cell, m-acon, Man (Taxonomy), MCOPCB1, Macrophages, i59, mac, membrane attack complex protein alphaM chain, hsp60, Glandular Epithelial Cells, Bone Marrow Derived Macrophages, Adenomatous Epithelial, Bone Marrow-Derived Macrophage, human, retina autoimmune disease, M-Acon, Bone Marrow-Derived, Mononuclear, lamellocyte, Mononuclear Leukocytes, Epithelial Cells, CG9244, Transitional Epithelial Cells, autoimmune disease of retina, Modern Man, Cells, mAc, Bone Marrow-Derived Macrophages, l(2)07054, granulocyte, FBgn0010100, liquid, histiocyte, mitochondrial adhaerens complex, Squamous Epithelial Cell, Glandular Epithelial Cell, terminal complement complex"],"name_synonyms":["Transitional Epithelial Cell, Transitional, macrophagocyte, SPH116, Squamous Cells, BEST:GH10550, Leukocyte, human being, microphthalmos bilateral, Epithelial Cell, Peripheral Blood Mononuclear Cells, Monocyte Derived Macrophages, typical plasmatocyte, AconM, Modern, mAcon, CG6457, Adenomatous, Microphthalmia with colobomatous cyst, Glandular Epithelial, Monocyte Derived, Squamous, Glandular, colobomatous orbital cyst, monocyte-derived macrophage, PBMC Peripheral Blood Mononuclear Cells, Transitional Epithelial, Cell, i204, Human, microphthalmia associated with colobomatous cyst, membrane attack complex protein beta2 chain, Mononuclear Leukocyte., Monocyte-Derived, ACON-1, Squamous Epithelial Cells, DmelCG6457, Cuboidal Glandular Epithelial Cells, Microphthalmia - anophthalmia - coloboma, Homo sapiens, macrophage, AIR, MAC, Monocyte-Derived Macrophage, SP116, Peripheral Blood Mononuclear Cell, TCC, Columnar Glandular Epithelial Cells, DmelCG9244, agranular plasmatocyte, Man, Squamous Epithelial, Monocyte-Derived Macrophages, Epithelial, Macrophage, average, Adenomatous Epithelial Cell, Peripheral Blood Human Mononuclear Cells, Adenomatous Epithelial Cells, Squamous Cell, m-acon, Man (Taxonomy), MCOPCB1, Macrophages, i59, mac, membrane attack complex protein alphaM chain, hsp60, Glandular Epithelial Cells, Bone Marrow Derived Macrophages, Adenomatous Epithelial, Bone Marrow-Derived Macrophage, human, retina autoimmune disease, M-Acon, Bone Marrow-Derived, Mononuclear, lamellocyte, Mononuclear Leukocytes, Epithelial Cells, CG9244, Transitional Epithelial Cells, autoimmune disease of retina, Modern Man, Cells, mAc, Bone Marrow-Derived Macrophages, l(2)07054, granulocyte, FBgn0010100, liquid, histiocyte, mitochondrial adhaerens complex, Squamous Epithelial Cell, Glandular Epithelial Cell, terminal complement complex"],"additional_accession":[]},"is_claimable":false,"name":"Primary normal human bronchial epithelial cells (NHBE) cultured at air liquid interface with or without macrophage cells (+/- Mac) derived from primary peripheral blood mononuclear cells","description":"Primary normal human bronchial epithelial cells (NHBE) cultured at air liquid interface with or without macrophage cells (+/- Mac) derived from primary peripheral blood mononuclear cells","dates":{"last_updated":"2025-09-24","first_public":"2020-09-04"},"accession":"PRJNA660617","cross_references":{"GEO":["GSE156727"],"taxon":["9606"]}}