ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/082/SRR12570082/SRR12570082_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/080/SRR12570080/SRR12570080_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/083/SRR12570083/SRR12570083_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/078/SRR12570078/SRR12570078_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/081/SRR12570081/SRR12570081.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/078/SRR12570078/SRR12570078.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/079/SRR12570079/SRR12570079_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/080/SRR12570080/SRR12570080_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/082/SRR12570082/SRR12570082_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/078/SRR12570078/SRR12570078_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/079/SRR12570079/SRR12570079_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/083/SRR12570083/SRR12570083_1.fastq.gzprimaryOK200Genomicsanhui universityhttps://www.ebi.ac.uk/ena/browser/view/PRJNA661030Homo sapiensIn previous study, we found circFAT1(e2) was highly expressed in breast cancer tissues and cell lines. And this factor is also a crucial role in breast cancer genesis and development. So in this study, we knocked down circFAT1(e2) in MCF-7 cells. The viability and metastasis was significantly inhibited with circFAT1(e2) depletion. In order to explore the potential down-stream gene of circFAT1(e2) in breast cancer cells. We extracted the total RNA from circFAT1(e2) knockdown MCF-7 cells and negative control group by using TriZol reagent. Then, toal RNA was subjected to transcriptome sequencing to determine the potential down-stream targets of circFAT1(e2). We expect to reveal the underlied mechanism of the regulatory effect on breast cancer of circFAT1(e2). Overall design: CircFAT1(e2) was knocked down in siZ5 group to identify the down-stream genes of this circRNA in MCF-7 cell, and siNC was used as a knockdown control in this experiment.ENAfalseTranscriptome analysis of gene expression in MCF-7 cell upon circFAT1(e2) depletionTranscriptome analysis of gene expression in MCF-7 cell upon circFAT1(e2) depletion2025-09-242023-01-27PRJNA661030GSE1573439606