ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/053/SRR12594053/SRR12594053.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/064/SRR12594064/SRR12594064.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/076/SRR12594076/SRR12594076.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/059/SRR12594059/SRR12594059.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/075/SRR12594075/SRR12594075.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/070/SRR12594070/SRR12594070.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/052/SRR12594052/SRR12594052.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/049/SRR12594049/SRR12594049.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/074/SRR12594074/SRR12594074.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/063/SRR12594063/SRR12594063.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/048/SRR12594048/SRR12594048.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/078/SRR12594078/SRR12594078.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/060/SRR12594060/SRR12594060.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/071/SRR12594071/SRR12594071.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/067/SRR12594067/SRR12594067.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/056/SRR12594056/SRR12594056.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/073/SRR12594073/SRR12594073.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/062/SRR12594062/SRR12594062.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/051/SRR12594051/SRR12594051.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/050/SRR12594050/SRR12594050.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/061/SRR12594061/SRR12594061.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/066/SRR12594066/SRR12594066.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/072/SRR12594072/SRR12594072.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/068/SRR12594068/SRR12594068.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/079/SRR12594079/SRR12594079.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/055/SRR12594055/SRR12594055.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/077/SRR12594077/SRR12594077.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/057/SRR12594057/SRR12594057.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/054/SRR12594054/SRR12594054.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/058/SRR12594058/SRR12594058.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/069/SRR12594069/SRR12594069.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR125/065/SRR12594065/SRR12594065.fastq.gzprimary200OKGenomicsInstitute for Cardiovascular Physiology, Goethe Universität Frankfurt am Mainhttps://www.ebi.ac.uk/ena/browser/view/PRJNA661550Homo sapiensxref:PubMed:33753848The vitamin D receptor (VDR) has been knocked out in monocytic THP-1 cells after stimulation of ko and control cells with the the natural VDR ligand 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), quadruplicate mRNA-seq has been performed Overall design: The knockout has been performed by lentiviral CRISPR/Cas9 gene editing using a guideRNA (gRNA) directed against the VDR gene. The control cells have been created using a non-targeting gRNA (NTC). Prior to MRNA-seq, the cells were treated for 24 h with 100 nM 1,25(OH)2D3 or the solvent control (EtOH).ENATHP-1 Cell Line, THP-1, NR1I1, Controlling, preventive therapy, VddrI, Pddr, CYP1, reference sample, Nr1i1, prophylaxis, PPP1R163, Cyp27b, Cell Lines, P450c1, VDDR, vdr-A, VDDRI, Nuclear receptor subfamily 1 group I member 1, xVDR, CYP27B, Cp2b, Cell, prevention, preventive measures, Cell Line, CP2B, VDR, Vdr, PDDR, mRNA-seq, control, CYP1alpha, Cells, 1, THP 1 Cell Line, Vdd1, VDD1, Vddr, THP-1 Cell Lines, Cyp1, 25-dihydroxyvitamin D3 receptor, THP 1 Cells, prevention and control, THP-1 Cell., Cyp40, ControlledTHP-1 Cell Line, THP-1, NR1I1, Controlling, preventive therapy, VddrI, Pddr, CYP1, reference sample, Nr1i1, prophylaxis, PPP1R163, Cyp27b, Cell Lines, P450c1, VDDR, vdr-A, VDDRI, Nuclear receptor subfamily 1 group I member 1, xVDR, CYP27B, Cp2b, Cell, prevention, preventive measures, Cell Line, CP2B, VDR, Vdr, PDDR, mRNA-seq, control, CYP1alpha, Cells, 1, THP 1 Cell Line, Vdd1, VDD1, Vddr, THP-1 Cell Lines, Cyp1, 25-dihydroxyvitamin D3 receptor, THP 1 Cells, prevention and control, THP-1 Cell., Cyp40, ControlledfalsemRNA-seq in VDR knockout and control THP-1 cellsmRNA-seq in VDR knockout and control THP-1 cells2025-09-242021-09-08PRJNA661550GSE157514960633753848