ENAGenomicsTranslational neurology, IKVL, Lund Universityhttps://www.ebi.ac.uk/ena/browser/view/PRJNA662593Homo sapiensxref:PubMed:32958605Myeloid-derived suppressor cells (MDSC) are known to contribute to immune evasion in cancer. However, the function of the human granulocytic (G)-MDSC subset during tumor progression is largely unknown, and there are no established markers for their identification in human tumor specimens. Using gene expression profiling, mass cytometry and tumor microarrays, we here demonstrate that human G-MDSCs occur as neutrophils at distinct maturation stages, with a disease-specific profile. G-MDSCs derived from patients with metastatic breast cancer (MBC) and malignant melanoma display a unique immature neutrophil profile, that is more similar to healthy donor neutrophils than to G-MDSCs from sepsis patients. Finally, we show that primary G-MDSCs from MBC patients co-transplanted with breast cancer cells, promote tumor growth and affect vessel formation, leading to myeloid immune cell exclusion. Our findings reveal a role for human G-MDSC in tumor progression and have clinical implications also for targeted immunotherapy. Overall design: Triplicate blood sample cells, per condition, were FACS sorted, RNA was extracted and hybridized to Affymetrix 1.0 ST arrays.ENAprimary breast cancer, malignant tumor of the breast, Band Cell, human being, Neoplasms, postnatal development, Benign Neoplasm, mammary neoplasm, Mammary Cancers, Human Mammary Neoplasms, growth and development, Tumor, Malignant, Polymorphonuclear, Human, Breast Malignant Tumor, Homo sapiens, "neoplasm of breast (disorder)" EXACT [SNOMEDCT_2005_07_31:126926005], Breast Tumor, "mammary tumor" EXACT [CSP2005:2016-0671], LE Cell, breast tumor, cancer of the breast, Man, Segmented/Neutrophils, Neutrophil Band Cells, BC, Mammary Neoplasms, malignant neoplasm of breast, Human Mammary, Man (Taxonomy), Malignancy, Mammary Neoplasm, Mammary Carcinoma, Human Mammary Carcinomas, PMNC, man, Cancer of the Breast, Neoplasias, Neutrophils, cancer of breast, Malignant Tumor of Breast, Mammary, Mammary Cancer, Malignancies, Breast, mammary cancer, LE, mammary tumor, Neutrophil, Cancer, Tumors, Breast Carcinoma, Human Mammary Neoplasm, Carcinoma, Leukocyte, Breast Carcinomas, Malignant Neoplasm, immune cell, "breast neoplasm" EXACT [MTH:120], Polymorphonuclear Neutrophils, Leukocytes, Modern, PMN cell, "breast tumor" EXACT [NCI2004_11_17:C2910], Cell, Polymorphonuclear Leukocyte, Breast Malignant Neoplasm, Neutrophil Band Cell, development, Benign, LE Cells, Mammary Carcinomas, Neoplasm, Human Mammary Carcinoma, NOS, Malignant Neoplasm of Breast, Carcinomas, Polymorphonuclear Neutrophil, Breast Neoplasm, leucocyte, distinct, growth pattern, non-developmental growth, postnatal growth, Benign Neoplasms, Cancers, Breast Malignant Neoplasms., "mammary neoplasm" RELATED [], human, NEUTSGNE, Malignant Neoplasms, white blood cell, breast cancer, Modern Man, Breast Tumors, Breast Cancer, Polymorphonuclear Leukocytes, Breast Malignant Tumors, Cancer of Breast, other neoplasm, growth, cancer, Neoplasia, breast, polymorphonuclear cellprimary breast cancer, malignant tumor of the breast, Band Cell, human being, Neoplasms, postnatal development, Benign Neoplasm, mammary neoplasm, Mammary Cancers, Human Mammary Neoplasms, growth and development, Tumor, Malignant, Polymorphonuclear, Human, Breast Malignant Tumor, Homo sapiens, "neoplasm of breast (disorder)" EXACT [SNOMEDCT_2005_07_31:126926005], Breast Tumor, "mammary tumor" EXACT [CSP2005:2016-0671], LE Cell, breast tumor, cancer of the breast, Man, Segmented/Neutrophils, Neutrophil Band Cells, BC, Mammary Neoplasms, malignant neoplasm of breast, Human Mammary, Man (Taxonomy), Malignancy, Mammary Neoplasm, Mammary Carcinoma, Human Mammary Carcinomas, PMNC, man, Cancer of the Breast, Neoplasias, Neutrophils, cancer of breast, Malignant Tumor of Breast, Mammary, Mammary Cancer, Malignancies, Breast, mammary cancer, LE, mammary tumor, Neutrophil, Cancer, Tumors, Breast Carcinoma, Human Mammary Neoplasm, Carcinoma, Leukocyte, Breast Carcinomas, Malignant Neoplasm, immune cell, "breast neoplasm" EXACT [MTH:120], Polymorphonuclear Neutrophils, Leukocytes, Modern, PMN cell, "breast tumor" EXACT [NCI2004_11_17:C2910], Cell, Polymorphonuclear Leukocyte, Breast Malignant Neoplasm, Neutrophil Band Cell, development, Benign, LE Cells, Mammary Carcinomas, Neoplasm, Human Mammary Carcinoma, NOS, Malignant Neoplasm of Breast, Carcinomas, Polymorphonuclear Neutrophil, Breast Neoplasm, leucocyte, distinct, growth pattern, non-developmental growth, postnatal growth, Benign Neoplasms, Cancers, Breast Malignant Neoplasms., "mammary neoplasm" RELATED [], human, NEUTSGNE, Malignant Neoplasms, white blood cell, breast cancer, Modern Man, Breast Tumors, Breast Cancer, Polymorphonuclear Leukocytes, Breast Malignant Tumors, Cancer of Breast, other neoplasm, growth, cancer, Neoplasia, breast, polymorphonuclear cellfalseHuman G-MDSCs are Neutrophils at Distinct Maturation Stages that Promote Tumor Growth and Immune Cell Exclusion in Breast CancerHuman G-MDSCs are Neutrophils at Distinct Maturation Stages that Promote Tumor Growth and Immune Cell Exclusion in Breast Cancer2025-09-242020-09-21PRJNA662593GSE157737960632958605