{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR126/092/SRR12621192/SRR12621192_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR126/093/SRR12621193/SRR12621193_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR126/091/SRR12621191/SRR12621191_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR126/093/SRR12621193/SRR12621193_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR126/092/SRR12621192/SRR12621192_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR126/091/SRR12621191/SRR12621191_1.fastq.gz"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Ren Ji Hospital, Shanghai Jiao Tong University"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA662736"],"scientific_name":["Homo sapiens"],"tag":["xref:PubMed:33391492"],"long_description":["Glioblastoma (GBM) is the most lethal primary brain tumor in adults with a median survival of around 15 months. A potential treatment strategy involves targeting glioma stem-like cells (GSCs) that are able to initiate, maintain, and repopulate the tumor mass. Here, we identify ACT001, a parthenolide derivative, targeting GSCs through regulation of adipocyte enhancer binding protein 1 (AEBP1) signaling.GSCs exhibit high response to ACT001 in compared with normal human astrocytes. AEBP1 is a putative target of ACT001 by RNA-Seq analysis, which expression associates with prognosis of GBM patients. Knockdown of AEBP1 inhibits GSC proliferation whereas ectopic expression of AEBP1 increases GSC proliferation and xenograft tumor growth. AEBP1 overexpression also attenuates ATC001-inhibited GSC orthotopic xenograft tumor growth. Treatment with ACT001 or PI3K inhibitor or AEBP1 depletion decreases AKT phosphorylation and GSC proliferation. However, constitutive AKT activation rescues ACT001 treatment or AEBP1 knockdown-inhibited cell proliferation. Additionally, ACT001 blocks TGF-β-activated AEBP1/AKT signaling in GSCs. ACT001 exhibits antitumor activity either as a single agent or in combination with SHP099, which provides significant survival benefits for GSC xenograft tumor -bearing animals. Overall design: Examination of differential gene expression in GSC 83 cells treated with or without ACT001 ."],"repository":["ENA"],"description_synonyms":["Glioma 1, CG5373, dP60, GLM, Vps34, ATVPS34, glial tumors, PI3K, Mixed Glioma, BcDNA:LD15217, Dp110, tumour of the neuroglia, Tumor, VPS34, 11621, AKT signalling cascade, PI-3 kinase, p110-alpha, PHOSPHATIDYLINOSITOL 3-KINASE, [M]Glioma NOS (morphologic abnormality), DmelCG2699, F8A5.4, tumor of the neuroglia, Scanning Transmission Electron Microscopy, [M]Gliomas (morphologic abnormality), p110alpha, 4, phosphatidylinositol 3-kinase activity, primary stem, parthenolide, Dp60, glioma (morphologic abnormality), Pi3K92D, CG4141, PI3K_59F, neuroglial tumour, catalyst activity, Neoplasm of the Neuroglia, Pi3k, PI3-kinase activity, AKT signaling cascade, PI[[3]]K, PI3Kgamma, MCAP, PKB signalling cascade, (1aR-(1aR*, p55alpha, tumour of neuroglia, PI3K 68D, Gliomas (morphologic abnormality), Pi3K, PI3k, protein kinase B signal transduction, glioma, MCMTC, neoplasm of the neuroglia, ATP - 1-phosphatidyl-1D-myo-inositol 3-phosphotransferase activity, Tumors, dVps34, Glioma (except Nasal glioma, PI3K68D, AKT signal transduction, P110DELTA, PtdIns-3-kinase activity, rea, 4E, p50alpha, obsolete_glioma, Malignant Glioma, CG2699, DmelCG5373, vacuolar protein sorting 34, dPIK, 1-phosphatidylinositol 3-kinase activity, Electron Microscopy, C530050K14, type-1 PI3K, protein kinase B signaling, Gliomas, Glial Cell Tumor, PKB signaling cascade., CG11621, p85alpha, Tumor of Neuroglia, PI3K 68_D, 7aS*, DmelCG4141, F8A5_4, 10bR*))-isomer, APDS, p110D, primary axis, Tumor of the Neuroglia, PI3KBETA, glial tumour, STEM, not neoplastic), Glioma, glial neoplasm, p120-PI3K, malignant (morphologic abnormality), Neoplasms of Neuroglia, droPIK57, Glioma NOS, PI3K92E, neuroglial tumor, 10aS*, Pi3K_59F, stalk, Neuroglial Tumor, PKB signal transduction, type I phosphatidylinositol kinase activity, PKB signaling, Malignant, phosphatidylinositol 3-kinase, PIK3, dVps34/PI3K59F, Pi3Kp60, 5-alpha-Epoxy-6-beta-hydroxygermacra-1(10), p60, PI3K-dp110, dPI3K, PHOSPATIDYLINOSITOL 3-KINASE, anon-92Ed, ACLP, Mixed Gliomas, Dp110/PI3K, PI3K-68D/E, class I, Glioma NOS (morphologic abnormality), Glial Neoplasm, PI3'K, Pi3Kp110, culm, neuroglial neoplasm, P110BETA, PI3K-68D, glial tumor, PI3K21B, [M]Gliomas, MCM, Tumors of Neuroglia, Cpk, dP110, protein kinase B signalling cascade, AKT signaling, 6330412C24Rik, AA414921, axis, p120, class II, malignant, type III phosphoinositide 3-kinase activity, p110, Vps34p, Scanning Transmission, Cell, PI(3)K, neoplasm of neuroglia, cpk, PI3K-92E/Dp110, PIK3C1, ATP:1-phosphatidyl-1D-myo-inositol 3-phosphotransferase activity, (-)-Parthenolide, vps34, CWS5, dp110, Mixed, [M]Glioma NOS, PI3CG, protein kinase B signaling cascade, PI-3-K, Glial Tumor, NOS (except Nasal glioma, PI3K-59F, Neuroglial Neoplasm, Neuroglial Neoplasms, PI3K-Dp110, Glial Cell, Malignant glioma, class III, 11(13)-dien-12-oic acid gamma-lactone, DmelCG11621, p110gamma, tumor of neuroglia, Neoplasm of Neuroglia, caPI3K, CLOVE, no ICD-O subtype, DmVps34, IMD14, PI3K_68D, PI3K-92D, Glial Cell Tumors, Malignant Gliomas, Dmp110"],"name_synonyms":["Glioma 1, CG5373, dP60, GLM, Vps34, ATVPS34, glial tumors, PI3K, Mixed Glioma, BcDNA:LD15217, Dp110, tumour of the neuroglia, Tumor, VPS34, 11621, AKT signalling cascade, PI-3 kinase, p110-alpha, PHOSPHATIDYLINOSITOL 3-KINASE, [M]Glioma NOS (morphologic abnormality), DmelCG2699, F8A5.4, tumor of the neuroglia, Scanning Transmission Electron Microscopy, [M]Gliomas (morphologic abnormality), p110alpha, 4, phosphatidylinositol 3-kinase activity, primary stem, parthenolide, Dp60, glioma (morphologic abnormality), Pi3K92D, CG4141, PI3K_59F, neuroglial tumour, catalyst activity, Neoplasm of the Neuroglia, Pi3k, PI3-kinase activity, AKT signaling cascade, PI[[3]]K, PI3Kgamma, MCAP, PKB signalling cascade, (1aR-(1aR*, p55alpha, tumour of neuroglia, PI3K 68D, Gliomas (morphologic abnormality), Pi3K, PI3k, protein kinase B signal transduction, glioma, MCMTC, neoplasm of the neuroglia, ATP - 1-phosphatidyl-1D-myo-inositol 3-phosphotransferase activity, Tumors, dVps34, Glioma (except Nasal glioma, PI3K68D, AKT signal transduction, P110DELTA, PtdIns-3-kinase activity, rea, 4E, p50alpha, obsolete_glioma, Malignant Glioma, CG2699, DmelCG5373, vacuolar protein sorting 34, dPIK, 1-phosphatidylinositol 3-kinase activity, Electron Microscopy, C530050K14, type-1 PI3K, protein kinase B signaling, Gliomas, Glial Cell Tumor, PKB signaling cascade., CG11621, p85alpha, Tumor of Neuroglia, PI3K 68_D, 7aS*, DmelCG4141, F8A5_4, 10bR*))-isomer, APDS, p110D, primary axis, Tumor of the Neuroglia, PI3KBETA, glial tumour, STEM, not neoplastic), Glioma, glial neoplasm, p120-PI3K, malignant (morphologic abnormality), Neoplasms of Neuroglia, droPIK57, Glioma NOS, PI3K92E, neuroglial tumor, 10aS*, Pi3K_59F, stalk, Neuroglial Tumor, PKB signal transduction, type I phosphatidylinositol kinase activity, PKB signaling, Malignant, phosphatidylinositol 3-kinase, PIK3, dVps34/PI3K59F, Pi3Kp60, 5-alpha-Epoxy-6-beta-hydroxygermacra-1(10), p60, PI3K-dp110, dPI3K, PHOSPATIDYLINOSITOL 3-KINASE, anon-92Ed, ACLP, Mixed Gliomas, Dp110/PI3K, PI3K-68D/E, class I, Glioma NOS (morphologic abnormality), Glial Neoplasm, PI3'K, Pi3Kp110, culm, neuroglial neoplasm, P110BETA, PI3K-68D, glial tumor, PI3K21B, [M]Gliomas, MCM, Tumors of Neuroglia, Cpk, dP110, protein kinase B signalling cascade, AKT signaling, 6330412C24Rik, AA414921, axis, p120, class II, malignant, type III phosphoinositide 3-kinase activity, p110, Vps34p, Scanning Transmission, Cell, PI(3)K, neoplasm of neuroglia, cpk, PI3K-92E/Dp110, PIK3C1, ATP:1-phosphatidyl-1D-myo-inositol 3-phosphotransferase activity, (-)-Parthenolide, vps34, CWS5, dp110, Mixed, [M]Glioma NOS, PI3CG, protein kinase B signaling cascade, PI-3-K, Glial Tumor, NOS (except Nasal glioma, PI3K-59F, Neuroglial Neoplasm, Neuroglial Neoplasms, PI3K-Dp110, Glial Cell, Malignant glioma, class III, 11(13)-dien-12-oic acid gamma-lactone, DmelCG11621, p110gamma, tumor of neuroglia, Neoplasm of Neuroglia, caPI3K, CLOVE, no ICD-O subtype, DmVps34, IMD14, PI3K_68D, PI3K-92D, Glial Cell Tumors, Malignant Gliomas, Dmp110"],"additional_accession":[]},"is_claimable":false,"name":"Targeting of glioma stem-like cells with a parthenolide derivative ACT001 through inhibition of AEBP1/PI3K/AKT signaling","description":"Targeting of glioma stem-like cells with a parthenolide derivative ACT001 through inhibition of AEBP1/PI3K/AKT signaling","dates":{"last_updated":"2025-09-24","first_public":"2020-09-12"},"accession":"PRJNA662736","cross_references":{"GEO":["GSE157779"],"taxon":["9606"],"PubMed":["33391492"]}}