{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR127/025/SRR12735025/SRR12735025_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR127/027/SRR12735027/SRR12735027_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR127/025/SRR12735025/SRR12735025_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR127/028/SRR12735028/SRR12735028_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR127/026/SRR12735026/SRR12735026_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR127/027/SRR12735027/SRR12735027_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR127/026/SRR12735026/SRR12735026_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR127/028/SRR12735028/SRR12735028_2.fastq.gz"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["nankai univesity"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA666208"],"scientific_name":["Homo sapiens"],"long_description":["Programmed death ligand-1 (PD-L1) is a well-known transmembrane protein, which antibodies present effective clinical therapy in multiple human cancers. However, the function of tumor cell-intrinsic PD-L1 and its related mechanism in breast cancer remains incompletely studied. Programmed death ligand 1 (PD-L1) on the membrane of tumor cells strengthens tumor immune escape. Tumor cell-intrinsic PD-L1 is also involved in tumorigenesis and development, but the mechanism in regulating PD-L1 expression remains incompletely studied. Here, we report a novel mechanism for PD-L1 that can be induced by hepatitis B X-interacting protein (HBXIP), an oncogenic transcriptional coactivator, promoting breast cancer growth. Overexpression of PD-L1 increases breast cancer proliferation in vitro and in vivo. Transcriptomic analysis also reveals that PD-L1 plays a critical role in cancer development. Furthermore, we find that the expression of PD-L1 is positively associated with HBXIP in breast cancer clinical tissues as well as in cell lines, PD-L1 and HBXIP expression have higher levels in tumor. Mechanistically, HBXIP predominantly stimulates the promoter activity of PD-L1 through coactivating transcription factor ETS2. Especially, HBXIP induced PD-L1 acetylation with the acetyltransferase p300 at lysine 270 (K270), enhancing PD-L1 protein stability. Functionally, depletion of HBXIP markedly attenuates PD-L1-induced breast tumor growth in vitro and in vivo. Moreover, aspirin decreased breast cancer growth via targeting PD-L1 and HBXIP. Taken together, our results extend a new mechanism of PD-L1 functions, expound non-immune effects of PD-L1 and imply broader uses for PD-L1 as a target in breast cancer therapy. Overall design: Examination of PD-L1 overexpression functions in breast cancer cells."],"tag":["xref:PubMed:33824459"],"repository":["ENA"],"description_synonyms":["Breast Carcinoma, Human Mammary Neoplasm, primary breast cancer, Carcinoma, malignant tumor of the breast, Breast Carcinomas, Malignant Neoplasm, \"breast neoplasm\" EXACT [MTH:120], postnatal development., 1110003H18Rik, Neoplasms, acetylation, Benign Neoplasm, mammary neoplasm, Mammary Cancers, Human Mammary Neoplasms, growth and development, Tumor, \"breast tumor\" EXACT [NCI2004_11_17:C2910], Malignant, Human, Breast Malignant Neoplasm, development, Breast Malignant Tumor, Benign, PDCD1LG1, PDCD1L1, \"neoplasm of breast (disorder)\" EXACT [SNOMEDCT_2005_07_31:126926005], Mammary Carcinomas, Breast Tumor, B7-H, Neoplasm, Human Mammary Carcinoma, \"mammary tumor\" EXACT [CSP2005:2016-0671], PDCD1 ligand 1, NOS, breast tumor, cancer of the breast, B7-H1, Malignant Neoplasm of Breast, activation, B7H1, Carcinomas, CD274, BC, B7 homolog 1, Mammary Neoplasms, malignant neoplasm of breast, Programmed death ligand 1, Human Mammary, Breast Neoplasm, Malignancy, growth pattern, Mammary Neoplasm, non-developmental growth, Mammary Carcinoma, HBXIP, postnatal growth, Human Mammary Carcinomas, Acetylations, Benign Neoplasms, Cancers, PD-L1, \"mammary neoplasm\" RELATED [], Cancer of the Breast, Malignant Neoplasms, Neoplasias, Hbxip, breast cancer, cancer of breast, Malignant Tumor of Breast, Mammary, Mammary Cancer, Breast Tumors, Breast Cancer, Breast Malignant Tumors, Cancer of Breast, Malignancies, Breast, mammary cancer, other neoplasm, cancer, mammary tumor, growth, Breast Malignant Neoplasms, Neoplasia, PDL1, breast, XIP, Cancer, Tumors"],"name_synonyms":["Breast Carcinoma, Human Mammary Neoplasm, primary breast cancer, Carcinoma, malignant tumor of the breast, Breast Carcinomas, Malignant Neoplasm, \"breast neoplasm\" EXACT [MTH:120], postnatal development., 1110003H18Rik, Neoplasms, acetylation, Benign Neoplasm, mammary neoplasm, Mammary Cancers, Human Mammary Neoplasms, growth and development, Tumor, \"breast tumor\" EXACT [NCI2004_11_17:C2910], Malignant, Human, Breast Malignant Neoplasm, development, Breast Malignant Tumor, Benign, PDCD1LG1, PDCD1L1, \"neoplasm of breast (disorder)\" EXACT [SNOMEDCT_2005_07_31:126926005], Mammary Carcinomas, Breast Tumor, B7-H, Neoplasm, Human Mammary Carcinoma, \"mammary tumor\" EXACT [CSP2005:2016-0671], PDCD1 ligand 1, NOS, breast tumor, cancer of the breast, B7-H1, Malignant Neoplasm of Breast, activation, B7H1, Carcinomas, CD274, BC, B7 homolog 1, Mammary Neoplasms, malignant neoplasm of breast, Programmed death ligand 1, Human Mammary, Breast Neoplasm, Malignancy, growth pattern, Mammary Neoplasm, non-developmental growth, Mammary Carcinoma, HBXIP, postnatal growth, Human Mammary Carcinomas, Acetylations, Benign Neoplasms, Cancers, PD-L1, \"mammary neoplasm\" RELATED [], Cancer of the Breast, Malignant Neoplasms, Neoplasias, Hbxip, breast cancer, cancer of breast, Malignant Tumor of Breast, Mammary, Mammary Cancer, Breast Tumors, Breast Cancer, Breast Malignant Tumors, Cancer of Breast, Malignancies, Breast, mammary cancer, other neoplasm, cancer, mammary tumor, growth, Breast Malignant Neoplasms, Neoplasia, PDL1, breast, XIP, Cancer, Tumors"],"additional_accession":[]},"is_claimable":false,"name":"Activation and acetylation of tumor-intrinsic PD-L1 via HBXIP accelerates breast cancer growth","description":"Activation and acetylation of tumor-intrinsic PD-L1 via HBXIP accelerates breast cancer growth","dates":{"last_updated":"2025-09-24","first_public":"2022-02-05"},"accession":"PRJNA666208","cross_references":{"GEO":["GSE158696"],"taxon":["9606"],"PubMed":["33824459"]}}