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for Human Neurophysiology and Genetics, South Australian Health and Medical Research Institute (SAHMRI)https://www.ebi.ac.uk/ena/browser/view/PRJNA667623Homo sapiensxref:PubMed:27698428Neural progenitors derived from human pluripotent stem cells (hPSCs) develop into electrophysiologically active neurons at heterogeneous rates, which can confound disease-relevant discoveries in neurology and psychiatry. The goal of this study was to resolve the diversity of functional states of hPSC-derived neurons in vitro by combining patch clamping with morphological and RNA-sequencing analysis of single neurons (Patch-seq). Overall design: Whole-transcriptome, single-cell mRNA-seq profiles of 56 patch-clamped and morphologically characterized cells (50 differentiated neurons and 6 astrocytes) generated using Smart-seq (SMARTer) chemistry.ENAdTAF[[II]]230, TAF[[II]]250, d230, human being, Modern, Nerve, TAF200, l(3)84Ab, dTAFII250, RNA-seq., BG:DS00004.13, TAFII-250, TAF250/230, EfW1, Cell, Human, dTAF230, dmTAF[[II]]230, TAFII250, iPSC, Homo sapiens, dmTAF1, Taf230, p230, TAF[[II]]250/230, TFIID, Whole Transcriptome Shotgun Sequencing, iPS cell, Man, TAF250, Taf[[II]]250, Taf200, Nerve Cells, dTAF[[II]]250, TAF[[II]]230, Man (Taxonomy), TFIID TAF250, cel, cell, Taf1p, TAF[II]250, CG17603, TAF[[II]], human, dTAF250, DmelCG17603, Taf250, SR3-5, Modern Man, Cells, Neuron, TAF, Nerve Cell, humans, TAF230, TAF1dTAF[[II]]230, TAF[[II]]250, d230, human being, Modern, Nerve, TAF200, l(3)84Ab, dTAFII250, RNA-seq., BG:DS00004.13, TAFII-250, TAF250/230, EfW1, Cell, Human, dTAF230, dmTAF[[II]]230, TAFII250, iPSC, Homo sapiens, dmTAF1, Taf230, p230, TAF[[II]]250/230, TFIID, Whole Transcriptome Shotgun Sequencing, iPS cell, Man, TAF250, Taf[[II]]250, Taf200, Nerve Cells, dTAF[[II]]250, TAF[[II]]230, Man (Taxonomy), TFIID TAF250, cel, cell, Taf1p, TAF[II]250, CG17603, TAF[[II]], human, dTAF250, DmelCG17603, Taf250, SR3-5, Modern Man, Cells, Neuron, TAF, Nerve Cell, humans, TAF230, TAF1falsePredicting the functional states of human iPSC-derived neurons with single-cell RNA-seq and electrophysiologyPredicting the functional states of human iPSC-derived neurons with single-cell RNA-seq and electrophysiology2025-09-242020-10-08PRJNA667623GSE159074960627698428