ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR127/088/SRR12782788/SRR12782788.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR127/089/SRR12782789/SRR12782789.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR127/087/SRR12782787/SRR12782787.fastq.gzprimaryOK200GenomicsUniversity of Kentuckyhttps://www.ebi.ac.uk/ena/browser/view/PRJNA667766Mus musculusxref:PubMed:35425699Prostate apoptosis response-4 (Par-4) is a tumor suppressor protein that is extensively investigated in cancer. The objective of this study is to determine the physiological function of Par-4 in normal cells. Our findings indicated that genetic loss of Par-4 in mice primarily results in adipocyte hypertrophy and obesity, and secondary leads to hepatic steatosis and insulin resistance. Moreover, we noted that Par-4 is downregulated in human subjects who are likely to become obese in the future, thereby serving as a predictor of obesity. Importantly, ChIP-Seq indicated that MDM2 is a target of Par-4 protein, which is known to function as a transcriptional coregulator. We show that Par-4 loss upregulates MDM2 target protein p53, and that p53 further induces complement factor C3 and its proteolytic fragment acylation stimulating protein (ASP), an adipokine that is known to be causally associated with obesity. These studies led to the identification of the Par-4-MDM2-p53-C3/ASP axis in regulation of obesity by Par-4. Overall design: We report potential gene targets of Par-4 upon sequencing of ChIP in 3T3-L1 using Par-4 antibody. Sequencing of pulled-down Par-4 antibody-bound chromatin.ENAnuclear chromatin, opsonin activity, dlkb1, cytoplasmic chromatin, 3T3 L1 Cells, par4, Lkb1, AA408040, CG9374, membrane bound, LKB1, par-4, STK11, B lymphocyte receptor complex., B-cell receptor complex, antibodies, 3T3-L1 Cell, Cell, Transcriptional repressor Par-4-like protein PAWR, DmelCG9374, Coagulation factor II receptor-like 3, NIH3T3-L1, antibody, immunoglobulin, BCR complex, B cell receptor accessory molecule complex, chromosome scaffold, Cells, PAR-4, R75140, Thrombin receptor-like 3, B cell receptor activity, 2310001G03Rik, PAR4, dLKB1, Par4, Prostate apoptosis response 4 protein, mLKB1, B-lymphocyte receptor complex, LKB1/PEUTZ JEGHERS KINASE, DmLKB1, anon-EST:Posey135, immunoglobulin complex, Chromatins, Par-4, 3T3-L1nuclear chromatin, opsonin activity, dlkb1, cytoplasmic chromatin, 3T3 L1 Cells, par4, Lkb1, AA408040, CG9374, membrane bound, LKB1, par-4, STK11, B lymphocyte receptor complex., B-cell receptor complex, antibodies, 3T3-L1 Cell, Cell, Transcriptional repressor Par-4-like protein PAWR, DmelCG9374, Coagulation factor II receptor-like 3, NIH3T3-L1, antibody, immunoglobulin, BCR complex, B cell receptor accessory molecule complex, chromosome scaffold, Cells, PAR-4, R75140, Thrombin receptor-like 3, B cell receptor activity, 2310001G03Rik, PAR4, dLKB1, Par4, Prostate apoptosis response 4 protein, mLKB1, B-lymphocyte receptor complex, LKB1/PEUTZ JEGHERS KINASE, DmLKB1, anon-EST:Posey135, immunoglobulin complex, Chromatins, Par-4, 3T3-L1falseChromatin Immunoprecipation Sequencing in 3T3-L1 preadipocytes using Par-4 antibodyChromatin Immunoprecipation Sequencing in 3T3-L1 preadipocytes using Par-4 antibody2025-09-242022-03-26PRJNA667766GSE1591471009035425699