ENAGenomicsChan Lab, Vascular Medicine Institute, University of Pittsburghhttps://www.ebi.ac.uk/ena/browser/view/PRJNA672710Homo sapiensBRD-K34222889 is a structural analog of the anti-inflammatory and senolytic drug piperlongumine. Piperlongumine inhibits GSTP1, an enzyme that places glutathione moieties on cysteine residues of various protein targets and for modulation of protein stability. The objective of this experiment was to determine the effect of a Piperlongumine analog BRD-K34222889 in endothelial dysfunction. Interestingly, BRD-K34222889 was found to inhibit glutathione S-transferase P (GSTP1), which increased ISCU protein stability via preventing glutathionylation and thus increased oxidative metabolism and decreased PAEC apoptosis. Activites of BRD-K34222889 reversed gene transcripts altered in PAECs with hypoxia that more than half of these genes were categorized in pathways relevant to cell cycle, cell death, and metabolism and all known to be controlled by ISCU. We use Affymetrix Clairom S platform to determine the whole gene expression. Overall design: HPAECs were induced with hypoxia with or without BRD-K34222889 and appropriate control for 48h. There were three biological replicate for each group for a total of 9 samples. The groups were Normoxia with vehicle, hypoxia with vehicle, hypoxia with BRD-K34222889xref:PubMed:34669463ENAtruncus pulmonalis, Profiling, Endothelial Cells, Lymphatic Endothelial., Transcriptome, human being, Capillary Endothelial Cells, Modern, Deficiencies, Monitorings, Gene, Capillary, Cell, Vascular Endothelial, Gene Expression Profilings, Human, Oxygen Deficiency, Oxygen Deficiencies, Gene Expression, Differential Display, Transcript Expression Analysis, transcription profiling, Anoxia, mRNA Differential Displays, Transcript Expression Analyses, Gene Expression Pattern Analysis, Homo sapiens, Lymphatic, Vascular, Artery, Gene Expression Monitorings, Lymphatic Endothelial Cell, Analysis, Profilings, Hypoxia, mRNA Differential Display, Man, gene expression profiling, Pulmonary, pulmonary arterial subtree, pulmonary arterial tree, Gene Expression Monitoring, Vascular Endothelial Cells, Man (Taxonomy), Transcriptome Profiling, Analyses, mRNA, Vascular Endothelial Cell, Transcriptome Profilings, Arteries, Hypoxemia, Transcriptomics, Differential Displays, Transcript Expression, Transcriptome Analysis, Endothelial, man, Lymphatic Endothelial Cells, Transcriptome Analyses, human, Monitoring, Pulmonary Arteries, Oxygen, arteria pulmonalis, Gene Expression Analysis, Capillary Endothelial, Lymphatic Endothelial, Deficiency, Gene Expression Analyses, Endothelial Cell, Modern Man, Cells, Expression Analysis, BRD, Capillary Endothelial Cell, Expression Analyses, Anoxemia, pulmonary arterial tree organ parttruncus pulmonalis, Profiling, Endothelial Cells, Lymphatic Endothelial., Transcriptome, human being, Capillary Endothelial Cells, Modern, Deficiencies, Monitorings, Gene, Capillary, Cell, Vascular Endothelial, Gene Expression Profilings, Human, Oxygen Deficiency, Oxygen Deficiencies, Gene Expression, Differential Display, Transcript Expression Analysis, transcription profiling, Anoxia, mRNA Differential Displays, Transcript Expression Analyses, Gene Expression Pattern Analysis, Homo sapiens, Lymphatic, Vascular, Artery, Gene Expression Monitorings, Lymphatic Endothelial Cell, Analysis, Profilings, Hypoxia, mRNA Differential Display, Man, gene expression profiling, Pulmonary, pulmonary arterial subtree, pulmonary arterial tree, Gene Expression Monitoring, Vascular Endothelial Cells, Man (Taxonomy), Transcriptome Profiling, Analyses, mRNA, Vascular Endothelial Cell, Transcriptome Profilings, Arteries, Hypoxemia, Transcriptomics, Differential Displays, Transcript Expression, Transcriptome Analysis, Endothelial, man, Lymphatic Endothelial Cells, Transcriptome Analyses, human, Monitoring, Pulmonary Arteries, Oxygen, arteria pulmonalis, Gene Expression Analysis, Capillary Endothelial, Lymphatic Endothelial, Deficiency, Gene Expression Analyses, Endothelial Cell, Modern Man, Cells, Expression Analysis, BRD, Capillary Endothelial Cell, Expression Analyses, Anoxemia, pulmonary arterial tree organ partfalseGene expression profiling with hypoxia and BRD-K34222889 from primary human pulmonary artery endothelial cells (HPAECs)Gene expression profiling with hypoxia and BRD-K34222889 from primary human pulmonary artery endothelial cells (HPAECs)2025-09-242021-10-28PRJNA672710GSE160255960634669463