ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/029/SRR12992029/SRR12992029_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/027/SRR12992027/SRR12992027_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/025/SRR12992025/SRR12992025_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/023/SRR12992023/SRR12992023_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/024/SRR12992024/SRR12992024_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/029/SRR12992029/SRR12992029_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/026/SRR12992026/SRR12992026_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/023/SRR12992023/SRR12992023_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/022/SRR12992022/SRR12992022_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/020/SRR12992020/SRR12992020_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/028/SRR12992028/SRR12992028_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/026/SRR12992026/SRR12992026_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/027/SRR12992027/SRR12992027_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/021/SRR12992021/SRR12992021_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/020/SRR12992020/SRR12992020_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/028/SRR12992028/SRR12992028_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/022/SRR12992022/SRR12992022_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/021/SRR12992021/SRR12992021_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/024/SRR12992024/SRR12992024_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/025/SRR12992025/SRR12992025_2.fastq.gzprimary200OKGenomicsDr. Stephen Lessnick, Center for Childhood Cancer, Nationwide Children's Hospitalhttps://www.ebi.ac.uk/ena/browser/view/PRJNA674797Homo sapiensThe purpose of this study was to compare the relevance of the FLI portion of EWS/FLI in the ability of the protein to act as a pioneer factor in Ewing sarcoma cells. EWS/FLI cDNA constructs including various deletions of the FLI portion were virally transduced into cells and we sought to determine the ATAC profile of each. Overall design: Endogenous EWS/FLI was knocked-down in the Ewing sarcoma A673 cell line and EWS/FLI cDNA constructs were expressed in these cells. Cells were collected after 8 days and ATAC was performed using Tn5 to detemrine the ability of each to EWS/FLI construct to alter chromatin state.ENAfalseThe FLI portion of EWS/FLI contributes a transcriptional regulatory function that is distinct from its DNA-binding function in Ewing sarcoma [ATAC-seq]The FLI portion of EWS/FLI contributes a transcriptional regulatory function that is distinct from its DNA-binding function in Ewing sarcoma [ATAC-seq]2025-09-242021-06-16PRJNA674797GSE1608959606