ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/041/SRR12992041/SRR12992041_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/037/SRR12992037/SRR12992037_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/045/SRR12992045/SRR12992045_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/030/SRR12992030/SRR12992030_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/035/SRR12992035/SRR12992035_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/042/SRR12992042/SRR12992042_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/038/SRR12992038/SRR12992038_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/039/SRR12992039/SRR12992039_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/036/SRR12992036/SRR12992036_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/042/SRR12992042/SRR12992042_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/043/SRR12992043/SRR12992043_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/037/SRR12992037/SRR12992037_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/040/SRR12992040/SRR12992040_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/039/SRR12992039/SRR12992039_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/034/SRR12992034/SRR12992034_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/033/SRR12992033/SRR12992033_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/031/SRR12992031/SRR12992031_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/030/SRR12992030/SRR12992030_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/035/SRR12992035/SRR12992035_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/043/SRR12992043/SRR12992043_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/031/SRR12992031/SRR12992031_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/036/SRR12992036/SRR12992036_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/045/SRR12992045/SRR12992045_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/032/SRR12992032/SRR12992032_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/033/SRR12992033/SRR12992033_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/034/SRR12992034/SRR12992034_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/044/SRR12992044/SRR12992044_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/032/SRR12992032/SRR12992032_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/038/SRR12992038/SRR12992038_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/040/SRR12992040/SRR12992040_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/044/SRR12992044/SRR12992044_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR129/041/SRR12992041/SRR12992041_2.fastq.gzprimaryOK200GenomicsDr. Stephen Lessnick, Center for Childhood Cancer, Nationwide Children's Hospitalhttps://www.ebi.ac.uk/ena/browser/view/PRJNA674798Homo sapiensThe purpose of this study was to compare the relevance of the FLI portion for genomic localization of the EWS/FLI protein in Ewing sarcoma cells. EWS/FLI cDNA constructs including various deletions of the FLI portion were virally transduced into cells and we sought to determine the ATAC profile of each. Overall design: Endogenous EWS/FLI was knocked-down in the Ewing sarcoma A673 cell line and EWS/FLI cDNA constructs were expressed in these cells. Cells were collected after 8 days and antibody-directed micrococcal nuclease was used to determine the binding locations of these EWS/FLI protein constructs. Two biological replicates for each sample were used to construct cDNA libraries each library was submitted twice to acheive sufficient read-depth and combined for analysis.ENAfalseThe FLI portion of EWS/FLI contributes a transcriptional regulatory function that is distinct from its DNA-binding function in Ewing sarcoma [CUT&RUN]The FLI portion of EWS/FLI contributes a transcriptional regulatory function that is distinct from its DNA-binding function in Ewing sarcoma [CUT&RUN]2025-09-242021-06-16PRJNA674798GSE1608969606