ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR130/029/SRR13020729/SRR13020729.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR130/025/SRR13020725/SRR13020725.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR130/021/SRR13020721/SRR13020721.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR130/026/SRR13020726/SRR13020726.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR130/022/SRR13020722/SRR13020722.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR130/024/SRR13020724/SRR13020724.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR130/023/SRR13020723/SRR13020723.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR130/027/SRR13020727/SRR13020727.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR130/030/SRR13020730/SRR13020730.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR130/031/SRR13020731/SRR13020731.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR130/028/SRR13020728/SRR13020728.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR130/032/SRR13020732/SRR13020732.fastq.gzprimaryOK200GenomicsPeeper, Molecular Oncology and Immunology, Netherlands Cancer Institutehttps://www.ebi.ac.uk/ena/browser/view/PRJNA675877Mus musculusWe found that Tmed10 regulates the cell-surface expression of PD-1 in T cells. We additionally show that treatment with AGN192403 can phenocopy Tmed10 KO by lysosomally degrading the Tmed10-PD-1 complex. Overall design: Here, we treated T cells (WT or Tmed10 KO) with AGN (100uM) or Veh (H2O) for 24 hours. The T cells were additionally activated with anti-CD3 antibodies (2.5ug/mL in PBS) for 24 hours (Act) or not (Rest).xref:PubMed:39510795ENAfalseTMED10 regulates cell-surface expression of PD-1TMED10 regulates cell-surface expression of PD-12025-09-242023-11-12PRJNA675877GSE1612111009039510795